BMRB Entry 11279
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11279
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Title: 2DZI/Solution Structure of the N-terminal Ubiquitin-like Domain in Human Ubiquitin-like Protein 4A (GDX)
Deposition date: 2010-08-09 Original release date: 2011-08-18
Authors: Zhao, C.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation: Zhao, C.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "2DZI/Solution Structure of the N-terminal Ubiquitin-like Domain in Human Ubiquitin-like Protein 4A (GDX)" . ., .-..
Assembly members:
ubiquitin-like domain, polymer, 81 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
ubiquitin-like domain: GSSGSSGMQLTVKALQGREC
SLQVPEDELVSTLKQLVSEK
LNVPVRQQRLLFKGKALADG
KRLSDYSIGPNSKLNLVVKP
L
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 347 |
| 15N chemical shifts | 81 |
| 1H chemical shifts | 571 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ubiquitin-like domain | 1 |
Entities:
Entity 1, ubiquitin-like domain 81 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | MET | GLN | LEU | ||||
| 2 | THR | VAL | LYS | ALA | LEU | GLN | GLY | ARG | GLU | CYS | ||||
| 3 | SER | LEU | GLN | VAL | PRO | GLU | ASP | GLU | LEU | VAL | ||||
| 4 | SER | THR | LEU | LYS | GLN | LEU | VAL | SER | GLU | LYS | ||||
| 5 | LEU | ASN | VAL | PRO | VAL | ARG | GLN | GLN | ARG | LEU | ||||
| 6 | LEU | PHE | LYS | GLY | LYS | ALA | LEU | ALA | ASP | GLY | ||||
| 7 | LYS | ARG | LEU | SER | ASP | TYR | SER | ILE | GLY | PRO | ||||
| 8 | ASN | SER | LYS | LEU | ASN | LEU | VAL | VAL | LYS | PRO | ||||
| 9 | LEU |
Samples:
sample_1: ubiquitin-like domain, [U-13C; U-15N], 1.18 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9748, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAG56766 |
| EMBL | CAH93235 |
| GB | AAA36790 AAA92650 AAH43346 AAH53589 ABZ10495 |
| REF | NP_001126901 NP_001164852 NP_001229350 NP_001253630 NP_055050 |
| SP | B0KWT6 B1MTV8 B7NZQ9 P11441 Q5R4T1 |
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