BMRB Entry 11282
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11282
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Title: Solution Structure of the UBA domain in Human Protein FAM100B
Deposition date: 2010-08-09 Original release date: 2011-08-18
Authors: Zhao, C.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation: Zhao, C.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the UBA domain in Human Protein FAM100B" . ., .-..
Assembly members:
UBA-like domain, residues 8-66, polymer, 66 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
UBA-like domain, residues 8-66: GSSGSSGMSVNMDELRHQVM
INQFVLAAGCAADQAKQLLQ
AAHWQFETALSTFFQETNIP
NSHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 234 |
| 15N chemical shifts | 63 |
| 1H chemical shifts | 373 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | UBA-like domain, residues 8-66 | 1 |
Entities:
Entity 1, UBA-like domain, residues 8-66 66 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | MET | SER | VAL | ||||
| 2 | ASN | MET | ASP | GLU | LEU | ARG | HIS | GLN | VAL | MET | ||||
| 3 | ILE | ASN | GLN | PHE | VAL | LEU | ALA | ALA | GLY | CYS | ||||
| 4 | ALA | ALA | ASP | GLN | ALA | LYS | GLN | LEU | LEU | GLN | ||||
| 5 | ALA | ALA | HIS | TRP | GLN | PHE | GLU | THR | ALA | LEU | ||||
| 6 | SER | THR | PHE | PHE | GLN | GLU | THR | ASN | ILE | PRO | ||||
| 7 | ASN | SER | HIS | HIS | HIS | HIS |
Samples:
sample_1: UBA-like domain, [U-13C; U-15N], 0.43 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9748, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| GB | AAH35511 AAH60859 ABW03765 EAW89383 EHA99413 |
| REF | NP_872371 XP_001151089 XP_002696214 XP_003340352 XP_003358005 |
| SP | Q8IYN6 |
| TPG | DAA18129 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
or all simulated shifts