BMRB Entry 11287
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11287
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Title: Solution structure of the C2H2 type zinc-binding domain of human zinc finger protein 292
Deposition date: 2010-08-09 Original release date: 2011-08-18
Authors: Yoneyama, M.; Tomizawa, T.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Yoneyama, M.; Tomizawa, T.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C2H2 type zinc-binding domain of human zinc finger protein 292" . ., .-..
Assembly members:
C2H2 type zinc-binding domain, polymer, 73 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
C2H2 type zinc-binding domain: GSSGSSGRKKPVSQSLEFPT
RYSPYRPYRCVHQGCFAAFT
IQQNLILHYQAVHKSDLPAF
SAEVEEESGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 297 |
| 15N chemical shifts | 62 |
| 1H chemical shifts | 450 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | C2H2 type zinc-binding domain | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, C2H2 type zinc-binding domain 73 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ARG | LYS | LYS | ||||
| 2 | PRO | VAL | SER | GLN | SER | LEU | GLU | PHE | PRO | THR | ||||
| 3 | ARG | TYR | SER | PRO | TYR | ARG | PRO | TYR | ARG | CYS | ||||
| 4 | VAL | HIS | GLN | GLY | CYS | PHE | ALA | ALA | PHE | THR | ||||
| 5 | ILE | GLN | GLN | ASN | LEU | ILE | LEU | HIS | TYR | GLN | ||||
| 6 | ALA | VAL | HIS | LYS | SER | ASP | LEU | PRO | ALA | PHE | ||||
| 7 | SER | ALA | GLU | VAL | GLU | GLU | GLU | SER | GLY | PRO | ||||
| 8 | SER | SER | GLY |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: C2H2 type zinc-binding domain, [U-13C; U-15N], 1.90 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; NTA 0.1 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9295, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA25456 BAB14654 BAI45414 |
| EMBL | CAD97823 |
| GB | AAB06954 AAH63594 AAI72360 AAV86070 AEB61065 |
| REF | NP_001179779 NP_055836 XP_001088554 XP_002806763 XP_002817165 |
| SP | O60281 |
| TPG | DAA26182 |
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