BMRB Entry 11299
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11299
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Title: Solution structure of the SH3 domain of the human Proline-serine-threonine phosphatase-interacting protein 1
Deposition date: 2010-08-09 Original release date: 2011-08-19
Authors: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the SH3 domain of the human Proline-serine-threonine phosphatase-interacting protein 1" . ., .-..
Assembly members:
SH3 domain, polymer, 69 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
SH3 domain: GSSGSSGAQEYRALYDYTAQ
NPDELDLSAGDILEVILEGE
DGWWTVERNGQRGFVPGSYL
EKLSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 256 |
| 15N chemical shifts | 65 |
| 1H chemical shifts | 403 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3 domain | 1 |
Entities:
Entity 1, SH3 domain 69 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ALA | GLN | GLU | ||||
| 2 | TYR | ARG | ALA | LEU | TYR | ASP | TYR | THR | ALA | GLN | ||||
| 3 | ASN | PRO | ASP | GLU | LEU | ASP | LEU | SER | ALA | GLY | ||||
| 4 | ASP | ILE | LEU | GLU | VAL | ILE | LEU | GLU | GLY | GLU | ||||
| 5 | ASP | GLY | TRP | TRP | THR | VAL | GLU | ARG | ASN | GLY | ||||
| 6 | GLN | ARG | GLY | PHE | VAL | PRO | GLY | SER | TYR | LEU | ||||
| 7 | GLU | LYS | LEU | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domian, [U-13C; U-15N], 1.07 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9736, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
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