BMRB Entry 11301
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11301
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Title: Solution structure of the Myb_DNA-binding domain of human Cell division cycle 5-like protein
Deposition date: 2010-08-09 Original release date: 2011-08-19
Authors: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Myb_DNA-binding domain of human Cell division cycle 5-like protein" . ., .-..
Assembly members:
Myb_DNA-binding domain, polymer, 66 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
Myb_DNA-binding domain: GSSGSSGKKTEWSREEEEKL
LHLAKLMPTQWRTIAPIIGR
TAAQCLEHYEFLLDKAAQRD
SGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 249 |
| 15N chemical shifts | 56 |
| 1H chemical shifts | 399 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Myb_DNA-binding domain | 1 |
Entities:
Entity 1, Myb_DNA-binding domain 66 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | LYS | LYS | THR | ||||
| 2 | GLU | TRP | SER | ARG | GLU | GLU | GLU | GLU | LYS | LEU | ||||
| 3 | LEU | HIS | LEU | ALA | LYS | LEU | MET | PRO | THR | GLN | ||||
| 4 | TRP | ARG | THR | ILE | ALA | PRO | ILE | ILE | GLY | ARG | ||||
| 5 | THR | ALA | ALA | GLN | CYS | LEU | GLU | HIS | TYR | GLU | ||||
| 6 | PHE | LEU | LEU | ASP | LYS | ALA | ALA | GLN | ARG | ASP | ||||
| 7 | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: Myb_DNA-binding domain, [U-13C; U-15N], 1.13 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9736, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
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