BMRB Entry 11306
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11306
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Title: Solution Structure of the Ring-H2 Finger Domain of Mouse Deltex Protein 2
Deposition date: 2010-08-10 Original release date: 2011-08-19
Authors: Miyamoto, K.; Muto, Y.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Miyamoto, K.; Muto, Y.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the Ring-H2 Finger Domain of Mouse Deltex Protein 2" . ., .-..
Assembly members:
Ring-H2 Finger Domain, polymer, 114 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
Ring-H2 Finger Domain: GSSGSSGEPEQVIRKYTEEL
KVAPEEDCIICMEKLAVASG
YSDMTDSKALGPMVVGRLTK
CSHAFHLLCLLAMYCNGNKD
GSLQCPSCKTIYGEKTGTQP
WGKMEVFRSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 465 |
| 15N chemical shifts | 107 |
| 1H chemical shifts | 733 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ring-H2 Finger Domain | 1 |
| 2 | ZINC ION no.1 | 2 |
| 3 | ZINC ION no.2 | 2 |
Entities:
Entity 1, Ring-H2 Finger Domain 114 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLU | PRO | GLU | ||||
| 2 | GLN | VAL | ILE | ARG | LYS | TYR | THR | GLU | GLU | LEU | ||||
| 3 | LYS | VAL | ALA | PRO | GLU | GLU | ASP | CYS | ILE | ILE | ||||
| 4 | CYS | MET | GLU | LYS | LEU | ALA | VAL | ALA | SER | GLY | ||||
| 5 | TYR | SER | ASP | MET | THR | ASP | SER | LYS | ALA | LEU | ||||
| 6 | GLY | PRO | MET | VAL | VAL | GLY | ARG | LEU | THR | LYS | ||||
| 7 | CYS | SER | HIS | ALA | PHE | HIS | LEU | LEU | CYS | LEU | ||||
| 8 | LEU | ALA | MET | TYR | CYS | ASN | GLY | ASN | LYS | ASP | ||||
| 9 | GLY | SER | LEU | GLN | CYS | PRO | SER | CYS | LYS | THR | ||||
| 10 | ILE | TYR | GLY | GLU | LYS | THR | GLY | THR | GLN | PRO | ||||
| 11 | TRP | GLY | LYS | MET | GLU | VAL | PHE | ARG | SER | GLY | ||||
| 12 | PRO | SER | SER | GLY |
Entity 2, ZINC ION no.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Ring-H2 Domain, [U-13C; U-15N], 1.05 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.1 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.880, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB18940 BAB18941 BAB28055 BAC31780 BAC35781 |
| GB | AAI66738 EDL19332 EDL19333 EDL19334 EDL19335 |
| REF | NP_001100627 NP_001243025 NP_001243026 NP_001243027 NP_076231 |
| SP | Q8R3P2 |
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