BMRB Entry 11309
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11309
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Title: Solution structure of the N-terminal zinc finger domain of mouse cell growth regulating nucleolar protein LYAR
Deposition date: 2010-08-10 Original release date: 2011-08-19
Authors: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Muto, Y.; Yokoyama, S.
Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Muto, Y.; Yokoyama, S.. "Solution structure of the N-terminal zinc finger domain of mouse cell growth regulating nucleolar protein LYAR" . ., .-..
Assembly members:
Zinc finger DNA binding domain, polymer, 79 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
Zinc finger DNA binding domain: GSSGSSGMVFFTCNACGESV
KKIQVEKHVSNCRNCECLSC
IDCGKDFWGDDYKSHVKCIS
EGQKYGGKGYEAKSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 299 |
| 15N chemical shifts | 72 |
| 1H chemical shifts | 452 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Zinc finger DNA binding domain | 1 |
| 2 | ZINC ION no.1 | 2 |
| 3 | ZINC ION no.2 | 2 |
Entities:
Entity 1, Zinc finger DNA binding domain 79 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | MET | VAL | PHE | ||||
| 2 | PHE | THR | CYS | ASN | ALA | CYS | GLY | GLU | SER | VAL | ||||
| 3 | LYS | LYS | ILE | GLN | VAL | GLU | LYS | HIS | VAL | SER | ||||
| 4 | ASN | CYS | ARG | ASN | CYS | GLU | CYS | LEU | SER | CYS | ||||
| 5 | ILE | ASP | CYS | GLY | LYS | ASP | PHE | TRP | GLY | ASP | ||||
| 6 | ASP | TYR | LYS | SER | HIS | VAL | LYS | CYS | ILE | SER | ||||
| 7 | GLU | GLY | GLN | LYS | TYR | GLY | GLY | LYS | GLY | TYR | ||||
| 8 | GLU | ALA | LYS | SER | GLY | PRO | SER | SER | GLY |
Entity 2, ZINC ION no.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: zinc finger DNA binding domain, [U-13C; U-15N], 1 mM; d-Tris-HCl 20 mM; NaCl 200 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 100 uM; H2O 90%; D2O 10%
condition_1: ionic strength: 220 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delagio, F. - processing
NMRView v5.0.4, Jhonson, B. A. - data analysis
Kujira v0.896, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB24554 BAC36458 |
| GB | AAB26644 AAI16924 AAI16928 EDL37545 EDL37546 |
| REF | NP_079557 XP_006503822 XP_006503823 XP_006503824 XP_006503825 |
| SP | Q08288 |
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