BMRB Entry 11318
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11318
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Title: Solution Strutcure of the SH3 domain of the Cdc42-interacting protein 4
Deposition date: 2010-08-10 Original release date: 2011-08-19
Authors: Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Strutcure of the SH3 domain of the Cdc42-interacting protein 4" . ., .-..
Assembly members:
SH3 domain, polymer, 70 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
SH3 domain: GSSGSSGGHCVAIYHFEGSS
EGTISMAEGEDLSLMEEDKG
DGWTRVRRKEGGEGYVPTSY
LRVTSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 257 |
| 15N chemical shifts | 61 |
| 1H chemical shifts | 389 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3 domain | 1 |
Entities:
Entity 1, SH3 domain 70 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLY | HIS | CYS | |
| 2 | VAL | ALA | ILE | TYR | HIS | PHE | GLU | GLY | SER | SER | |
| 3 | GLU | GLY | THR | ILE | SER | MET | ALA | GLU | GLY | GLU | |
| 4 | ASP | LEU | SER | LEU | MET | GLU | GLU | ASP | LYS | GLY | |
| 5 | ASP | GLY | TRP | THR | ARG | VAL | ARG | ARG | LYS | GLU | |
| 6 | GLY | GLY | GLU | GLY | TYR | VAL | PRO | THR | SER | TYR | |
| 7 | LEU | ARG | VAL | THR | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1.36 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.925, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAD96829 BAE00629 BAF80144 BAG36103 BAK63850 |
| EMBL | CAA04062 CAG38751 CAH90516 |
| GB | AAC41729 AAH13002 AAI33400 AAM46851 AAP35344 |
| REF | NP_001074999 NP_001125273 NP_001275891 NP_004231 XP_001092276 |
| SP | Q15642 Q5RCJ1 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts