BMRB Entry 11327
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11327
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Title: Solution structure of the B-box domain of the human tripartite motif-containing 63 protein
Deposition date: 2010-08-10 Original release date: 2011-08-19
Authors: Miyamoto, K.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Miyamoto, K.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the B-box domain of the human tripartite motif-containing 63 protein" . ., .-..
Assembly members:
zf-B box, polymer, 64 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
zf-B box: GSSGSSGHPMCKEHEDEKIN
IYCLTCEVPTCSMCKVFGIH
KACEVAPLQSVFQGQKTESG
PSSG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 243 |
15N chemical shifts | 54 |
1H chemical shifts | 377 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | zf-B box | 1 |
2 | ZINC ION no.1 | 2 |
3 | ZINC ION no.2 | 2 |
Entities:
Entity 1, zf-B box 64 residues - Formula weight is not available
1 | GLY | SER | SER | GLY | SER | SER | GLY | HIS | PRO | MET | ||||
2 | CYS | LYS | GLU | HIS | GLU | ASP | GLU | LYS | ILE | ASN | ||||
3 | ILE | TYR | CYS | LEU | THR | CYS | GLU | VAL | PRO | THR | ||||
4 | CYS | SER | MET | CYS | LYS | VAL | PHE | GLY | ILE | HIS | ||||
5 | LYS | ALA | CYS | GLU | VAL | ALA | PRO | LEU | GLN | SER | ||||
6 | VAL | PHE | GLN | GLY | GLN | LYS | THR | GLU | SER | GLY | ||||
7 | PRO | SER | SER | GLY |
Entity 2, ZINC ION no.1 - Zn - 65.409 Da.
1 | ZN |
Samples:
sample_1: zf-B_box, [U-13C; U-15N], 2.09 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9321, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 700 MHz
Related Database Links:
PDB | |
DBJ | BAB71318 BAG60157 |
EMBL | CAC33173 CAC81706 |
GB | AAH80529 AAI12722 AAK39519 AAP36037 AAP36890 |
REF | NP_001039760 NP_001171685 NP_115977 XP_002811293 XP_002811294 |
SP | Q969Q1 |
TPG | DAA32118 |
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