BMRB Entry 11329
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11329
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Title: Solution structure of the Bromodomain of human SWI/SNF related matrix associated actin dependent regulator of cromatin subfamily A member 2
Deposition date: 2010-08-10 Original release date: 2011-08-19
Authors: Yoneyama, M.; Kigawa, T.; Saito, K.; Tochio, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Yoneyama, M.; Kigawa, T.; Saito, K.; Tochio, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the Bromodomain of human SWI/SNF related matrix associated actin dependent regulator of cromatin subfamily A member 2" . ., .-..
Assembly members:
Bromodomain, polymer, 123 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
Bromodomain: GSSGSSGSPNPPKLTKQMNA
IIDTVINYKDSSGRQLSEVF
IQLPSRKELPEYYELIRKPV
DFKKIKERIRNHKYRSLGDL
EKDVMLLCHNAQTFNLEGSQ
IYEDSIVLQSVFKSARQSGP
SSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 528 |
| 15N chemical shifts | 115 |
| 1H chemical shifts | 847 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Bromodomain | 1 |
Entities:
Entity 1, Bromodomain 123 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | SER | PRO | ASN | ||||
| 2 | PRO | PRO | LYS | LEU | THR | LYS | GLN | MET | ASN | ALA | ||||
| 3 | ILE | ILE | ASP | THR | VAL | ILE | ASN | TYR | LYS | ASP | ||||
| 4 | SER | SER | GLY | ARG | GLN | LEU | SER | GLU | VAL | PHE | ||||
| 5 | ILE | GLN | LEU | PRO | SER | ARG | LYS | GLU | LEU | PRO | ||||
| 6 | GLU | TYR | TYR | GLU | LEU | ILE | ARG | LYS | PRO | VAL | ||||
| 7 | ASP | PHE | LYS | LYS | ILE | LYS | GLU | ARG | ILE | ARG | ||||
| 8 | ASN | HIS | LYS | TYR | ARG | SER | LEU | GLY | ASP | LEU | ||||
| 9 | GLU | LYS | ASP | VAL | MET | LEU | LEU | CYS | HIS | ASN | ||||
| 10 | ALA | GLN | THR | PHE | ASN | LEU | GLU | GLY | SER | GLN | ||||
| 11 | ILE | TYR | GLU | ASP | SER | ILE | VAL | LEU | GLN | SER | ||||
| 12 | VAL | PHE | LYS | SER | ALA | ARG | GLN | SER | GLY | PRO | ||||
| 13 | SER | SER | GLY |
Samples:
sample_1: Bromodomain, [U-13C; U-15N], 1.39 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; Guanidine oxalate 75 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 195 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9318, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA05142 BAB27925 BAC04280 BAE90149 BAG60343 |
| EMBL | CAA62832 CAH89596 |
| GB | AAH22975 AAH56199 AAH75641 AAI42522 AAI56186 |
| REF | NP_001092585 NP_001124708 NP_001276326 NP_001276327 NP_001276328 |
| SP | Q6DIC0 |
| TPG | DAA26902 |
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