BMRB Entry 11340
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11340
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Title: Solution structure of the RING domain of the human TNF receptor-associated factor 6 protein
Deposition date: 2010-08-10 Original release date: 2011-08-19
Authors: Miyamoto, K.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation: Miyamoto, K.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the RING domain of the human TNF receptor-associated factor 6 protein" . ., .-..
Assembly members:
RING domain, polymer, 86 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
RING domain: GSSGSSGMEEIQGYDVEFDP
PLESKYECPICLMALREAVQ
TPCGHRFCKACIIKSIRDAG
HKCPVDNEILLENQLFPDNF
AKREIL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 373 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 584 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RING domain | 1 |
| 2 | ZINC ION no.1 | 2 |
| 3 | ZINC ION no.2 | 2 |
Entities:
Entity 1, RING domain 86 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | MET | GLU | GLU | ||||
| 2 | ILE | GLN | GLY | TYR | ASP | VAL | GLU | PHE | ASP | PRO | ||||
| 3 | PRO | LEU | GLU | SER | LYS | TYR | GLU | CYS | PRO | ILE | ||||
| 4 | CYS | LEU | MET | ALA | LEU | ARG | GLU | ALA | VAL | GLN | ||||
| 5 | THR | PRO | CYS | GLY | HIS | ARG | PHE | CYS | LYS | ALA | ||||
| 6 | CYS | ILE | ILE | LYS | SER | ILE | ARG | ASP | ALA | GLY | ||||
| 7 | HIS | LYS | CYS | PRO | VAL | ASP | ASN | GLU | ILE | LEU | ||||
| 8 | LEU | GLU | ASN | GLN | LEU | PHE | PRO | ASP | ASN | PHE | ||||
| 9 | ALA | LYS | ARG | GLU | ILE | LEU |
Entity 2, ZINC ION no.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: RING domain, [U-13C; U-15N], 1.08 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9820, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| BMRB | 15014 |
| PDB | |
| GB | AAI02523 ABC47877 ABD72516 ABF06558 AFG21868 |
| REF | NP_001029833 XP_004016459 XP_004264064 XP_004330939 XP_004627096 |
| SP | Q3ZCC3 |
| TPG | DAA21840 |
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