BMRB Entry 11345
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11345
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Title: Solution structure of the SWIRM domain of baker's yeast Transcriptional adapter 2
Deposition date: 2010-08-10 Original release date: 2011-08-19
Authors: Yoneyama, M.; Tochio, N.; Koshiba, S.; Tomizawa, T.; Watanabe, S.; Harada, T.; Umehara, T.; Tanaka, A.; Kigawa, T.; Yokoyama, S.
Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Tomizawa, T.; Watanabe, S.; Harada, T.; Umehara, T.; Tanaka, A.; Kigawa, T.; Yokoyama, S.. "Solution structure of the SWIRM domain of baker's yeast Transcriptional adapter 2" . ., .-..
Assembly members:
SWIRM, polymer, 88 residues, Formula weight is not available
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
SWIRM: GSSGSSGNMTISDIQHAPDY
ALLSNDEQQLCIQLKILPKP
YLVLKEVMFRELLKTGGNLS
KSACRELLNIDPIKANRIYD
FFQSQNWM
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 382 |
| 15N chemical shifts | 87 |
| 1H chemical shifts | 627 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SWIRM | 1 |
Entities:
Entity 1, SWIRM 88 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ASN | MET | THR | ||||
| 2 | ILE | SER | ASP | ILE | GLN | HIS | ALA | PRO | ASP | TYR | ||||
| 3 | ALA | LEU | LEU | SER | ASN | ASP | GLU | GLN | GLN | LEU | ||||
| 4 | CYS | ILE | GLN | LEU | LYS | ILE | LEU | PRO | LYS | PRO | ||||
| 5 | TYR | LEU | VAL | LEU | LYS | GLU | VAL | MET | PHE | ARG | ||||
| 6 | GLU | LEU | LEU | LYS | THR | GLY | GLY | ASN | LEU | SER | ||||
| 7 | LYS | SER | ALA | CYS | ARG | GLU | LEU | LEU | ASN | ILE | ||||
| 8 | ASP | PRO | ILE | LYS | ALA | ASN | ARG | ILE | TYR | ASP | ||||
| 9 | PHE | PHE | GLN | SER | GLN | ASN | TRP | MET |
Samples:
sample_1: SWIRM, [U-13C; U-15N], 0.3 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
TOPSPIN v1.3, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9820, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE2 900 MHz
Related Database Links:
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