BMRB Entry 11346
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11346
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Title: Solution structure of Fn14 CRD domain PubMed: 19241374
Deposition date: 2010-09-07 Original release date: 2011-09-07
Authors: He, F.; Dang, W.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.
Citation: He, Fahu; Dang, Weirong; Saito, Kohei; Watanabe, Satoru; Kobayashi, Naohiro; Guntert, Peter; Kigawa, Takanori; Tanaka, Akiko; Muto, Yutaka; Yokoyama, Shigeyuki. "Solution structure of the cysteine-rich domain in Fn14, a member of the tumor necrosis factor receptor superfamily." Protein Sci. 18, 650-656 (2009).
Assembly members:
CRD domain, UNP residues 28-70, polymer, 50 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
CRD domain, UNP residues 28-70: GSSGSSGEQAPGTAPCSRGS
SWSADLDKCMDCASCRARPH
SDFCLGCAAA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 161 |
| 15N chemical shifts | 40 |
| 1H chemical shifts | 242 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CRD domain, UNP residues 28-70 | 1 |
Entities:
Entity 1, CRD domain, UNP residues 28-70 50 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLU | GLN | ALA | |
| 2 | PRO | GLY | THR | ALA | PRO | CYS | SER | ARG | GLY | SER | |
| 3 | SER | TRP | SER | ALA | ASP | LEU | ASP | LYS | CYS | MET | |
| 4 | ASP | CYS | ALA | SER | CYS | ARG | ALA | ARG | PRO | HIS | |
| 5 | SER | ASP | PHE | CYS | LEU | GLY | CYS | ALA | ALA | ALA |
Samples:
sample_1: Fn14, [U-13C; U-15N], 1.1 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | condition_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | condition_1 |
Software:
CYANA v2.1, P.GUNTERT, ET, AL. - refinement, structure solution
NMRPipe v20031121, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer, and, Bax - processing
NMRView v5.0.4, Johnson, One, Moon, Scientific - data analysis
xwinnmr v3.5, Bruker, Biospin - collection
Kujira v0.9839, Kobayashi N. - data analysis
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| BMRB | 17237 |
| PDB | |
| DBJ | BAA94792 BAJ20302 |
| GB | AAF69108 AAH02718 ABM83894 ABM87215 EAW85429 |
| REF | NP_057723 XP_001089176 XP_001165479 XP_002826080 XP_003269240 |
| SP | Q9NP84 |
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