BMRB Entry 11349
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11349
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of LIM domain in LIM-protein 3
Deposition date: 2010-09-07 Original release date: 2011-09-07
Authors: He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.
Citation: He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.. "Solution structure of LIM domain in LIM-protein 3" . ., .-..
Assembly members:
LIM domain, polymer, 72 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
LIM domain: GSSGSSGCQECKKTIMPGTR
KMEYKGSSWHETCFICHRCQ
QPIGTKSFIPKDNQNFCVPC
YEKQHASGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 289 |
| 15N chemical shifts | 66 |
| 1H chemical shifts | 437 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LIM domain | 1 |
| 2 | ZINC ION no.1 | 2 |
| 3 | ZINC ION no.2 | 2 |
Entities:
Entity 1, LIM domain 72 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | CYS | GLN | GLU | ||||
| 2 | CYS | LYS | LYS | THR | ILE | MET | PRO | GLY | THR | ARG | ||||
| 3 | LYS | MET | GLU | TYR | LYS | GLY | SER | SER | TRP | HIS | ||||
| 4 | GLU | THR | CYS | PHE | ILE | CYS | HIS | ARG | CYS | GLN | ||||
| 5 | GLN | PRO | ILE | GLY | THR | LYS | SER | PHE | ILE | PRO | ||||
| 6 | LYS | ASP | ASN | GLN | ASN | PHE | CYS | VAL | PRO | CYS | ||||
| 7 | TYR | GLU | LYS | GLN | HIS | ALA | SER | GLY | PRO | SER | ||||
| 8 | SER | GLY |
Entity 2, ZINC ION no.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: LIM domain, [U-13C; U-15N], 0.8 mM; d-Tris-HCl 20 mM; NaCl 100 mM; ZnCl2 100 uM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121, Delaglio F. - processing
NMRView v5.0.4, Johnson B.A. - data analysis
Kujira v0.863, Kobayashi N. - data analysis
CYANA v2.0.17, Guntert P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts