BMRB Entry 11351
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11351
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Title: Solution structure of the NEUZ (NHR) domain in Neuralized from Drosophila melanogaster
Deposition date: 2010-09-07 Original release date: 2011-09-07
Authors: He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Tarada, T.; Yokoyama, S.
Citation: He, F.; Saito, K.; Kobayashi, N.; Harada, T.; Watanabe, S.; Kigawa, T.; Guntert, P.; Ohara, O.; Tanaka, A.; Unzai, S.; Muto, Y.; Yokoyama, S.. "Structural and functional characterization of the NHR1 domain of the Drosophila neuralized E3 ligase in the notch signaling pathway." J. Mol. Biol. ., .-..
Assembly members:
NEUZ(NHR) domain, polymer, 168 residues, Formula weight is not available
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
NEUZ(NHR) domain: GSSGSSGPLQFHSVHGDNIR
ISRDGTLARRFESFCRAITF
SARPVRINERICVKFAEISN
NWNGGIRFGFTSNDPVTLEG
TLPKYACPDLTNRPGFWAKA
LHEQYCEKDNILYYYVNGAG
DVIYGINNEEKGVILTGIDT
RSLLWTVIDIYGNCTGIEFL
DSRIYMYQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 733 |
| 15N chemical shifts | 173 |
| 1H chemical shifts | 1144 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NEUZ(NHR) domain | 1 |
Entities:
Entity 1, NEUZ(NHR) domain 168 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | LEU | GLN | ||||
| 2 | PHE | HIS | SER | VAL | HIS | GLY | ASP | ASN | ILE | ARG | ||||
| 3 | ILE | SER | ARG | ASP | GLY | THR | LEU | ALA | ARG | ARG | ||||
| 4 | PHE | GLU | SER | PHE | CYS | ARG | ALA | ILE | THR | PHE | ||||
| 5 | SER | ALA | ARG | PRO | VAL | ARG | ILE | ASN | GLU | ARG | ||||
| 6 | ILE | CYS | VAL | LYS | PHE | ALA | GLU | ILE | SER | ASN | ||||
| 7 | ASN | TRP | ASN | GLY | GLY | ILE | ARG | PHE | GLY | PHE | ||||
| 8 | THR | SER | ASN | ASP | PRO | VAL | THR | LEU | GLU | GLY | ||||
| 9 | THR | LEU | PRO | LYS | TYR | ALA | CYS | PRO | ASP | LEU | ||||
| 10 | THR | ASN | ARG | PRO | GLY | PHE | TRP | ALA | LYS | ALA | ||||
| 11 | LEU | HIS | GLU | GLN | TYR | CYS | GLU | LYS | ASP | ASN | ||||
| 12 | ILE | LEU | TYR | TYR | TYR | VAL | ASN | GLY | ALA | GLY | ||||
| 13 | ASP | VAL | ILE | TYR | GLY | ILE | ASN | ASN | GLU | GLU | ||||
| 14 | LYS | GLY | VAL | ILE | LEU | THR | GLY | ILE | ASP | THR | ||||
| 15 | ARG | SER | LEU | LEU | TRP | THR | VAL | ILE | ASP | ILE | ||||
| 16 | TYR | GLY | ASN | CYS | THR | GLY | ILE | GLU | PHE | LEU | ||||
| 17 | ASP | SER | ARG | ILE | TYR | MET | TYR | GLN |
Samples:
sample_1: NEUZ(NHR) domain, [U-13C; U-15N], 1.0 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9820, Kobayashi, N. - data analysis
CYANA v2.1, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
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