BMRB Entry 11353
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11353
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Title: Solution structure of PHD domain in inhibitor of growth protein 3 (ING3)
Deposition date: 2010-09-07 Original release date: 2011-09-07
Authors: He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.
Citation: He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.. "Solution structure of PHD domain in inhibitor of growth protein 3 (ING3)" . ., .-..
Assembly members:
PHD domain, polymer, 70 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
PHD domain: GSSGSSGYCICNQVSYGEMV
GCDNQDCPIEWFHYGCVGLT
EAPKGKWYCPQCTAAMKRRG
SRHKSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 265 |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 399 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PHD domain | 1 |
| 2 | ZINC ION no.1 | 2 |
| 3 | ZINC ION no.2 | 2 |
Entities:
Entity 1, PHD domain 70 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | TYR | CYS | ILE | |
| 2 | CYS | ASN | GLN | VAL | SER | TYR | GLY | GLU | MET | VAL | |
| 3 | GLY | CYS | ASP | ASN | GLN | ASP | CYS | PRO | ILE | GLU | |
| 4 | TRP | PHE | HIS | TYR | GLY | CYS | VAL | GLY | LEU | THR | |
| 5 | GLU | ALA | PRO | LYS | GLY | LYS | TRP | TYR | CYS | PRO | |
| 6 | GLN | CYS | THR | ALA | ALA | MET | LYS | ARG | ARG | GLY | |
| 7 | SER | ARG | HIS | LYS | SER | GLY | PRO | SER | SER | GLY |
Entity 2, ZINC ION no.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: PHD domain, [U-13C; U-15N], 0.8 mM; d-Tris-HCl 20 mM; NaCl 100 mM; ZnCl2 0.1 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121, Delaglio F. - processing
NMRView v5.0.4, Johnson B.A. - data analysis
Kujira v0.863, Kobayashi N. - data analysis
CYANA v2.0.17, Guntert P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAA90942 BAC38021 BAE43273 BAG57123 BAG73291 |
| EMBL | CAF90810 CAG31907 CAH90959 CAJ83008 CDQ78875 |
| GB | AAG12172 AAG23285 AAG23286 AAH05721 AAH18342 |
| REF | NP_001008672 NP_001025904 NP_001029279 NP_001080280 NP_001125551 |
| SP | Q498T3 Q5RBA1 Q5ZK36 Q66KD5 Q7ZX31 |
| TPG | DAA30453 |
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