BMRB Entry 11357
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11357
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Title: Solution structure of WW domain in transcription elongation regulator 1
Deposition date: 2010-09-07 Original release date: 2011-09-07
Authors: He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.
Citation: He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.. "Solution structure of WW domain in transcription elongation regulator 1" . ., .-..
Assembly members:
WW domain, polymer, 73 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
WW domain: GSSGSSGKAKPVATAPIPGT
PWCVVWTGDERVFFYNPTTR
LSMWDRPDDLIGRADVDKII
QEPPHKKSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 295 |
| 15N chemical shifts | 61 |
| 1H chemical shifts | 454 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | WW domain | 1 |
Entities:
Entity 1, WW domain 73 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | LYS | ALA | LYS | ||||
| 2 | PRO | VAL | ALA | THR | ALA | PRO | ILE | PRO | GLY | THR | ||||
| 3 | PRO | TRP | CYS | VAL | VAL | TRP | THR | GLY | ASP | GLU | ||||
| 4 | ARG | VAL | PHE | PHE | TYR | ASN | PRO | THR | THR | ARG | ||||
| 5 | LEU | SER | MET | TRP | ASP | ARG | PRO | ASP | ASP | LEU | ||||
| 6 | ILE | GLY | ARG | ALA | ASP | VAL | ASP | LYS | ILE | ILE | ||||
| 7 | GLN | GLU | PRO | PRO | HIS | LYS | LYS | SER | GLY | PRO | ||||
| 8 | SER | SER | GLY |
Samples:
sample_1: WW domain, [U-13C; U-15N], 0.8 mM; phosphate buffer NA 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121, Delaglio F. - processing
NMRView v5.0.4, Johnson B.A. - data analysis
Kujira v0.9321, Kobayashi N. - data analysis
CYANA v2.0.17, Guntert P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
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