BMRB Entry 11364
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11364
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Title: Solution structure of the first SURP domain of human splicing factor SF3a120
Deposition date: 2010-09-07 Original release date: 2011-09-07
Authors: Kuwasako, K.; He, F.; Inoue, M.; Tanaka, A.; Sugano, S.; Guntert, P.; Muto, Y.; Yokoyama, S.
Citation: Kuwasako, K.; He, F.; Inoue, M.; Tanaka, A.; Sugano, S.; Guntert, P.; Muto, Y.; Yokoyama, S.. "Solution structures of the SURP domains and the subunit-assembly mechanism within the splicing factor SF3a complex in 17S U2 snRNP" Structure ., .-..
Assembly members:
SURP domain, polymer, 64 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: Escherichia coli
Entity Sequences (FASTA):
SURP domain: GEVRNIVDKTASFVARNGPE
FEARIRQNEINNPKFNFLNP
NDPYHAYYRHKVSEFKEGKA
QEPS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 291 |
| 15N chemical shifts | 66 |
| 1H chemical shifts | 449 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SURP domain | 1 |
Entities:
Entity 1, SURP domain 64 residues - Formula weight is not available
| 1 | GLY | GLU | VAL | ARG | ASN | ILE | VAL | ASP | LYS | THR | ||||
| 2 | ALA | SER | PHE | VAL | ALA | ARG | ASN | GLY | PRO | GLU | ||||
| 3 | PHE | GLU | ALA | ARG | ILE | ARG | GLN | ASN | GLU | ILE | ||||
| 4 | ASN | ASN | PRO | LYS | PHE | ASN | PHE | LEU | ASN | PRO | ||||
| 5 | ASN | ASP | PRO | TYR | HIS | ALA | TYR | TYR | ARG | HIS | ||||
| 6 | LYS | VAL | SER | GLU | PHE | LYS | GLU | GLY | LYS | ALA | ||||
| 7 | GLN | GLU | PRO | SER |
Samples:
sample_1: SURP domain, [U-13C; U-15N], 2.0 mM; soldium phosphate 20 mM; d-DTT 1 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 20 mM; pH: 7.0; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9321, Kobayashi, N. - data analysis
CYANA v2.1, Guntert, P. - refinement, structure solution
OPALP v1.4, Koradi, R. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 700 MHz
- Bruker AVANCE 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts