BMRB Entry 11456
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11456
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Title: Solution structure of the N-terminal dsRBD from RNA helicase A PubMed: 22454253
Deposition date: 2011-11-29 Original release date: 2012-08-29
Authors: Nagata, Takashi; Muto, Yutaka; Tsuda, Kengo; Inoue, Makoto; Kigawa, Takanori; Terada, Takaho; Shirouzu, Mikako; Yokoyama, Shigeyuki
Citation: Nagata, Takashi; Tsuda, Kengo; Kobayashi, Naohiro; Shirouzu, Mikako; Kigawa, Takanori; Guntert, Peter; Yokoyama, Shigeyuki; Muto, Yutaka. "Solution structures of the double-stranded RNA-binding domains from RNA helicase A" Proteins 80, 1699-1706 (2012).
Assembly members:
entity, polymer, 99 residues, 10733.203 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: E. coli - cell free
Entity Sequences (FASTA):
entity: GSSGSSGIKNFLYAWCGKRK
MTPAYEIRAVGNKNRQKFMC
EVRVEGFNYAGMGNSTNKKD
AQSNAARDFVNYLVRINEVK
SEEVPAVGIVPPPSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 384 |
| 15N chemical shifts | 104 |
| 1H chemical shifts | 622 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RNA helicase A | 1 |
Entities:
Entity 1, RNA helicase A 99 residues - 10733.203 Da.
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ILE | LYS | ASN | ||||
| 2 | PHE | LEU | TYR | ALA | TRP | CYS | GLY | LYS | ARG | LYS | ||||
| 3 | MET | THR | PRO | ALA | TYR | GLU | ILE | ARG | ALA | VAL | ||||
| 4 | GLY | ASN | LYS | ASN | ARG | GLN | LYS | PHE | MET | CYS | ||||
| 5 | GLU | VAL | ARG | VAL | GLU | GLY | PHE | ASN | TYR | ALA | ||||
| 6 | GLY | MET | GLY | ASN | SER | THR | ASN | LYS | LYS | ASP | ||||
| 7 | ALA | GLN | SER | ASN | ALA | ALA | ARG | ASP | PHE | VAL | ||||
| 8 | ASN | TYR | LEU | VAL | ARG | ILE | ASN | GLU | VAL | LYS | ||||
| 9 | SER | GLU | GLU | VAL | PRO | ALA | VAL | GLY | ILE | VAL | ||||
| 10 | PRO | PRO | PRO | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: entity, [U-100% 13C; U-100% 15N], 1.3 mM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm, Guntert, Mumenthaler and Wuthrich - refinement, structure solution
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 700 MHz
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB28848 BAE24846 BAE41336 |
| GB | AAC05301 AAC05725 AAH33911 AAH89159 AAI69284 |
| REF | NP_001100654 NP_031868 XP_005363931 XP_005363932 XP_006250057 |
| SP | O70133 |
Download simulated HSQC data in one of the following formats:
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