BMRB Entry 11486
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11486
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Title: Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
Deposition date: 2012-03-07 Original release date: 2013-03-12
Authors: Tochio, Naoya; Umehara, Takashi; Kigawa, Takanori; Yokoyama, Shigeyuki
Citation: Tochio, Naoya; Umehara, Takashi; Kigawa, Takanori; Yokoyama, Shigeyuki. "Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT" Not known ., .-..
Assembly members:
entity_1, polymer, 37 residues, 4078.767 Da.
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: E. coli - cell free
Entity Sequences (FASTA):
entity_1: GSSGSSGEKFACDYCSFTCL
SKGHLKVHIERVHKKIK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 148 |
| 15N chemical shifts | 28 |
| 1H chemical shifts | 210 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, entity_1 37 residues - 4078.767 Da.
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLU | LYS | PHE | ||||
| 2 | ALA | CYS | ASP | TYR | CYS | SER | PHE | THR | CYS | LEU | ||||
| 3 | SER | LYS | GLY | HIS | LEU | LYS | VAL | HIS | ILE | GLU | ||||
| 4 | ARG | VAL | HIS | LYS | LYS | ILE | LYS |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: protein, [U-13C; U-15N], 1.09 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; zinc chloride 50 uM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
xwinnmr, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - data analysis
Kujira, N. Kobayashi - data analysis
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 900 MHz
Related Database Links:
| PDB |
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