BMRB Entry 15038
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15038
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Title: 1H, 13C, and 15N resonance assignments of FK506-binding domain of Plasmodium falciparum FKBP35 PubMed: 19636818
Deposition date: 2006-11-19 Original release date: 2007-06-27
Authors: Kang, Congbao; Ye, Hong; Yoon, Hye Rim; Yoon, Ho Sup
Citation: Kang, Cong Bao; Ye, Hong; Yoon, Hye Rim; Yoon, Ho Sup. "1H, 13C, and 15N resonance assignments of FK506-binding domain of Plasmodium falciparum FKBP35" Biomol. NMR Assignments 1, 27-28 (2007).
Assembly members:
pfFKBP35FKBD, polymer, 135 residues, 14141 Da.
Natural source: Common Name: Plasmodium falciparum Taxonomy ID: 5833 Superkingdom: Eukaryota Kingdom: not available Genus/species: Plasmodium falciparum
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
pfFKBP35FKBD: MTTEQEFEKVELTADGGVIK
TILKKGDEGEENIPKKGNEV
TVHYVGKLESTGKVFDSSFD
RNVPFKFHLEQGEVIKGWDI
CVSSMRKNEKCLVRIESMYG
YGDEGCGESIPGNSVLLFEI
ELLSFRELEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 514 |
| 15N chemical shifts | 129 |
| 1H chemical shifts | 883 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | pfFKBP35FKBD | 1 |
Entities:
Entity 1, pfFKBP35FKBD 135 residues - 14141 Da.
| 1 | MET | THR | THR | GLU | GLN | GLU | PHE | GLU | LYS | VAL | ||||
| 2 | GLU | LEU | THR | ALA | ASP | GLY | GLY | VAL | ILE | LYS | ||||
| 3 | THR | ILE | LEU | LYS | LYS | GLY | ASP | GLU | GLY | GLU | ||||
| 4 | GLU | ASN | ILE | PRO | LYS | LYS | GLY | ASN | GLU | VAL | ||||
| 5 | THR | VAL | HIS | TYR | VAL | GLY | LYS | LEU | GLU | SER | ||||
| 6 | THR | GLY | LYS | VAL | PHE | ASP | SER | SER | PHE | ASP | ||||
| 7 | ARG | ASN | VAL | PRO | PHE | LYS | PHE | HIS | LEU | GLU | ||||
| 8 | GLN | GLY | GLU | VAL | ILE | LYS | GLY | TRP | ASP | ILE | ||||
| 9 | CYS | VAL | SER | SER | MET | ARG | LYS | ASN | GLU | LYS | ||||
| 10 | CYS | LEU | VAL | ARG | ILE | GLU | SER | MET | TYR | GLY | ||||
| 11 | TYR | GLY | ASP | GLU | GLY | CYS | GLY | GLU | SER | ILE | ||||
| 12 | PRO | GLY | ASN | SER | VAL | LEU | LEU | PHE | GLU | ILE | ||||
| 13 | GLU | LEU | LEU | SER | PHE | ARG | GLU | LEU | GLU | HIS | ||||
| 14 | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: pfFKBP35FKBD, [U-13C; U-15N], 0.5 mM; Na-PO4 20 mM; NaCl 50 mM; DTT 1 mM; EDTA 0.1 mM; D2O 10%; H2O 90%
sample_conditions_1: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCC(CO)NH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CC(CO)NH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| (H)CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v1.3, Bruker Biospin - collection
NMRPipe v2.3, F Delaglio, S Grzesiek, GW Vuister, G Zhu, J Pfeifer and A Bax - processing
NMRView v5.2.2, B Johnson, One Moon Scientific - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB |
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