BMRB Entry 15058
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15058
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Title: 1H assignment of En-6 PubMed: 17663000
Deposition date: 2006-11-29 Original release date: 2007-10-16
Authors: Pedrini, Bill; Placzek, William; Koculi, Eda; Alimenti, Claudio; LaTerza, Antonietta; Luporini, Pierangelo; Wuthrich, Kurt
Citation: Pedrini, Bill; Placzek, William; Koculi, Eda; Alimenti, Claudio; LaTerza, Antonietta; Luporini, Pierangelo; Wuthrich, Kurt. "Cold-adaptation in sea-water-borne signal proteins: Sequence and NMR structure of the pheromone En-6 from the Antarctic ciliate Euplotes nobilii" J. Mol. Biol. 372, 277-286 (2007).
Assembly members:
En-6, polymer, 63 residues, Formula weight is not available
Natural source: Common Name: Euplotes nobilii Taxonomy ID: 184062 Superkingdom: Eukaryota Kingdom: not available Genus/species: Euplotes nobilii
Experimental source: Production method: purified from the natural source Host organism: Euplotes nobilii
Entity Sequences (FASTA):
En-6: TDPEEHFDPNTNCDYTNSQD
AWDYCTNYIVNSSCGEICCN
DCFDETGTGACRAQAFGNSC
LNW
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 344 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | En-6 monomer | 1 |
Entities:
Entity 1, En-6 monomer 63 residues - Formula weight is not available
| 1 | THR | ASP | PRO | GLU | GLU | HIS | PHE | ASP | PRO | ASN | ||||
| 2 | THR | ASN | CYS | ASP | TYR | THR | ASN | SER | GLN | ASP | ||||
| 3 | ALA | TRP | ASP | TYR | CYS | THR | ASN | TYR | ILE | VAL | ||||
| 4 | ASN | SER | SER | CYS | GLY | GLU | ILE | CYS | CYS | ASN | ||||
| 5 | ASP | CYS | PHE | ASP | GLU | THR | GLY | THR | GLY | ALA | ||||
| 6 | CYS | ARG | ALA | GLN | ALA | PHE | GLY | ASN | SER | CYS | ||||
| 7 | LEU | ASN | TRP |
Samples:
sample_1: En-6 1 ± 0.2 mM; sodium phosphate 20 ± 0.5 mM
sample_2: En-6 1 ± 0.2 mM; sodium phosphate 20 ± 0.5 mM
sample_3: En-6 1 ± 0.2 mM; sodium phosphate 20 ± 0.5 mM; DSS 1 ± 0.1 mM
sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
| 1D 1H | sample_3 | isotropic | sample_conditions_1 |
Software:
CARA v1.5.3, Keller, R - chemical shift assignment
XWINNMR, Bruker Biospin - collection
ATNOS-CANDID v1.2, Guntert, Mumenthaler and Wuthrich, Herrmann, Guntert and Wuthrich - peak assignment, peak picking, structure solution
OPALp, Koradi, Billeter and Guntert - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - peak assignment, peak picking, structure solution
NMR spectrometers:
- Bruker Avance 600 MHz