BMRB Entry 15072

Name: OSCP-NT (1-120) in complex with N-terminal (1-25) alpha subunit from F1-ATPase
Published: 2007-04-17

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PDB ID: 2jmx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15072
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: OSCP-NT (1-120) in complex with N-terminal (1-25) alpha subunit from F1-ATPase PubMed: 17355883

Deposition date: 2006-12-12 Original release date: 2007-04-17

Authors: Carbajo, Rodrigo; Neuhaus, David; Kellas, Fiona; Yang, Ji-Chun; Runswick, Michael; Montgomery, Martin; Walker, John

Citation: Carbajo, Rodrigo; Kellas, Fiona; Yang, Ji-Chun; Runswick, Michael; Montgomery, Martin; Walker, John; Neuhaus, David. "How the N-terminal Domain of the OSCP Subunit of Bovine F1Fo-ATP Synthase Interacts with the N-terminal Region of an Alpha Subunit" J. Mol. Biol. 368, 310-318 (2007).

Assembly members:
oscp-nt, polymer, 120 residues, Formula weight is not available
alpha-nt, polymer, 25 residues, Formula weight is not available

Natural source: Common Name: Cow Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
oscp-nt: FAKLVRPPVQIYGIEGRYAT ALYSAASKQNKLEQVEKELL RVGQILKEPKMAASLLNPYV KRSVKVKSLSDMTAKEKFSP LTSNLINLLAENGRLTNTPA VISAFSTMMSVHRGEVPCTV
alpha-nt: QKTGTAEVSSILEERILGAD TSVDL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	222
15N chemical shifts	100
1H chemical shifts	738

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	OSCP N-terminal 1-120	1
2	alpha N-terminal 1-25	2

Entities:

Entity 1, OSCP N-terminal 1-120 120 residues - Formula weight is not available

1	PHE	ALA	LYS	LEU	VAL	ARG	PRO	PRO	VAL	GLN
2	ILE	TYR	GLY	ILE	GLU	GLY	ARG	TYR	ALA	THR
3	ALA	LEU	TYR	SER	ALA	ALA	SER	LYS	GLN	ASN
4	LYS	LEU	GLU	GLN	VAL	GLU	LYS	GLU	LEU	LEU
5	ARG	VAL	GLY	GLN	ILE	LEU	LYS	GLU	PRO	LYS
6	MET	ALA	ALA	SER	LEU	LEU	ASN	PRO	TYR	VAL
7	LYS	ARG	SER	VAL	LYS	VAL	LYS	SER	LEU	SER
8	ASP	MET	THR	ALA	LYS	GLU	LYS	PHE	SER	PRO
9	LEU	THR	SER	ASN	LEU	ILE	ASN	LEU	LEU	ALA
10	GLU	ASN	GLY	ARG	LEU	THR	ASN	THR	PRO	ALA
11	VAL	ILE	SER	ALA	PHE	SER	THR	MET	MET	SER
12	VAL	HIS	ARG	GLY	GLU	VAL	PRO	CYS	THR	VAL

Entity 2, alpha N-terminal 1-25 25 residues - Formula weight is not available

1

GLN

LYS

THR

GLY

THR

ALA

GLU

VAL

SER

2

ILE

LEU

GLU

ARG

ILE

LEU

GLY

ALA

ASP

3

THR

SER

VAL

ASP

LEU

Samples:

sample_1: oscp-nt, [U-99% 13C; U-99% 15N], 0.5 mM; alpha-nt 1.5 mM

sample_conditions_1: ionic strength: 0.5 M; pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

SPARKY, Goddard - chemical shift assignment

X-PLOR, Brunger - structure solution

NMR spectrometers:

Bruker Avance 800 MHz
Bruker DMX 600 MHz
Bruker DRX 500 MHz

PDB	2BO5 2JMX 4B2Q 5ARA 5ARE 5ARH 5ARI 5FIJ 5FIK 5FIL 1BMF 1E1Q 1E1R 1E79 1H8E 1H8H 1NBM 1OHH 1QO1 1W0J 1W0K 2CK3 2JDI 2JMX 2W6E 2W6F 2W6G 2W6H 2W6I 2W6J 2WSS 4ASU 4TSF 4TT3
GB	AAA30676 AAI02205 ELR58089 AAB59266 AAI16060 ACD02421 EFB21996 EGW04262
PRF	1002210A
REF	NP_776669 XP_005674722 XP_005893414 XP_005956973 XP_005956974 NP_001172071 NP_001268629 NP_001274083 NP_777109 XP_001506958
SP	P13621 P19483 P80021
TPG	DAA33707 DAA15776
DBJ	BAK09231
EMBL	CAA45865

Biological Magnetic Resonance Data Bank