BMRB Entry 15098
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15098
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Title: SOLUTION STRUCTURES OF THE BRK DOMAINS OF THE HUMAN CHROMO HELICASE DOMAIN 7 AND 8, REVEALS STRUCTURAL SIMILARITY WITH GYF DOMAIN SUGGESTING A ROLE IN PROTEIN INTERACTION
Deposition date: 2007-01-09 Original release date: 2012-08-03
Authors: AB, E.; De Jong, R.; Diercks, T.; Xiaoyun, J.; Daniels, M.; Kaptein, R.; Folkers, G.
Citation: AB, E.; De Jong, R.; Diercks, T.; Xiaoyun, J.; Daniels, M.; Kaptein, R.; Folkers, G.. "Solution Structures of the Brk Domains of the Human Chromo Helicase Domain 7 and 8, Reveals Structural Similarity with Gyf Domain Suggesting a Role in Protein Interaction" . ., .-..
Assembly members:
CHROMODOMAIN-HELICASE-DNA-BINDING_PROTEIN_8, polymer, 95 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI
Entity Sequences (FASTA):
CHROMODOMAIN-HELICASE-DNA-BINDING_PROTEIN_8: MGSSHHHHHHSSHMLVPRGS
LKKLVELEVECMEEPNHLDV
DLETRIPVINKVDGTLLVGE
DAPRRAELEMWLQGHPEFAV
DPRFLAYMEDRRKQK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 241 |
| 15N chemical shifts | 53 |
| 1H chemical shifts | 380 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 8 | 1 |
Entities:
Entity 1, CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 8 95 residues - Formula weight is not available
1991-2011 His Tag 2012-2085 BRK DOMANI
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | HIS | MET | LEU | VAL | PRO | ARG | GLY | SER | ||||
| 3 | LEU | LYS | LYS | LEU | VAL | GLU | LEU | GLU | VAL | GLU | ||||
| 4 | CYS | MET | GLU | GLU | PRO | ASN | HIS | LEU | ASP | VAL | ||||
| 5 | ASP | LEU | GLU | THR | ARG | ILE | PRO | VAL | ILE | ASN | ||||
| 6 | LYS | VAL | ASP | GLY | THR | LEU | LEU | VAL | GLY | GLU | ||||
| 7 | ASP | ALA | PRO | ARG | ARG | ALA | GLU | LEU | GLU | MET | ||||
| 8 | TRP | LEU | GLN | GLY | HIS | PRO | GLU | PHE | ALA | VAL | ||||
| 9 | ASP | PRO | ARG | PHE | LEU | ALA | TYR | MET | GLU | ASP | ||||
| 10 | ARG | ARG | LYS | GLN | LYS |
Samples:
sample_1: CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 8, [U-13C; U-15N], 0.8 mM; sodium phosphate 50 mM; sodium chloride 150 mM; sodium azide 0.02%
sample_conditions_1: ionic strength: 150 mM; pH: 7.0; pressure: 1.0 atm; temperature: 298.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCO | sample_1 | isotropic | sample_conditions_1 |
| HNCACO | sample_1 | isotropic | sample_conditions_1 |
| HNCACB | sample_1 | isotropic | sample_conditions_1 |
| CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| HBHACONH | sample_1 | isotropic | sample_conditions_1 |
| HNCAHA | sample_1 | isotropic | sample_conditions_1 |
| CCCONH | sample_1 | isotropic | sample_conditions_1 |
| CCH-COSY | sample_1 | isotropic | sample_conditions_1 |
| HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| HNH-NOESY | sample_1 | isotropic | sample_conditions_1 |
| HCH-NOESY | sample_1 | isotropic | sample_conditions_1 |
| CNH-NOESY | sample_1 | isotropic | sample_conditions_1 |
| HH-NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS FOR WATER REFINEMENT vREFINEMENT, BRUNGER,ADAMS,CLORE,DELANO,GROS, GROSSE-KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,READ,RICE,SIMONSON,WARREN - refinement
SPARKY, T Goddard - structure solution
NMR spectrometers:
- Bruker DMX 700 MHz
Related Database Links:
| PDB |
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