BMRB Entry 15125
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15125
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Title: N.N. PubMed: 18815416
Deposition date: 2007-02-01 Original release date: 2008-10-27
Authors: Lin, Y.; Padmanabhan, B.; Yokoyama, S.; Guntert, P.
Citation: Lin, Yi-Jan; Umehara, Takashi; Inoue, Makoto; Saito, Kohei; Kigawa, Takanori; Jang, Moon-Kyoo; Ozato, Keiko; Yokoyama, Shigeyuki; Padmanabhan, Balasundaram; Guntert, Peter. "Solution structure of the extraterminal domain of the bromodomain-containing protein BRD4" Protein Sci. 17, 2174-2179 (2008).
Assembly members:
Brd4-ET domain, polymer, 90 residues, 10328.770 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis Host organism: Escherichia coli
Entity Sequences (FASTA):
Brd4-ET domain: GSSGSSGESEEEDKCKPMSY
EEKRQLSLDINKLPGEKLGR
VVHIIQSREPSLKNSNPDEI
EIDFETLKPSTLRELERYVT
SCLRKKRKPQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 387 |
| 15N chemical shifts | 87 |
| 1H chemical shifts | 625 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Brd4-ET domain | 1 |
Entities:
Entity 1, Brd4-ET domain 90 residues - 10328.770 Da.
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLU | SER | GLU | |
| 2 | GLU | GLU | ASP | LYS | CYS | LYS | PRO | MET | SER | TYR | |
| 3 | GLU | GLU | LYS | ARG | GLN | LEU | SER | LEU | ASP | ILE | |
| 4 | ASN | LYS | LEU | PRO | GLY | GLU | LYS | LEU | GLY | ARG | |
| 5 | VAL | VAL | HIS | ILE | ILE | GLN | SER | ARG | GLU | PRO | |
| 6 | SER | LEU | LYS | ASN | SER | ASN | PRO | ASP | GLU | ILE | |
| 7 | GLU | ILE | ASP | PHE | GLU | THR | LEU | LYS | PRO | SER | |
| 8 | THR | LEU | ARG | GLU | LEU | GLU | ARG | TYR | VAL | THR | |
| 9 | SER | CYS | LEU | ARG | LYS | LYS | ARG | LYS | PRO | GLN |
Samples:
sample_1: Brd4-ET domain, [U-100% 13C; U-100% 15N], 1.05 mM; sodium chloride 100 mM; TRIS, [U-2H], 20 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%
sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-SEPARATED NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 13C-SEPARATED NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.2.2, P.GUNTERT ET AL. - refinement, structure solution
OPALP v1.4, R.KORADI ET AL. - refinement
NMRView, B.A.JOHNSON - data analysis
Kujira, N.KOBAYASHI - data analysis
NMR spectrometers:
- BRUKER DRX 600 MHz
- BRUKER AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAE27987 BAE72981 |
| EMBL | CAA66186 CAA72780 |
| GB | AAC27978 AAG02191 AAH35266 AAI38835 AAI38836 |
| REF | NP_001094373 NP_001191701 NP_001273559 NP_055114 NP_065254 |
| SP | O60885 Q9ESU6 |
| TPG | DAA28223 |
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