BMRB Entry 15142
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15142
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Title: Solution structure of dynein light chain 2A
Deposition date: 2007-02-24 Original release date: 2009-10-15
Authors: Kouno, T.; Nabeshima, Y.; Mizuguchi, M.; Kawano, K.
Citation: Kouno, T.; Nabeshima, Y.; Mizuguchi, M.; Kawano, K.. "Solution structure of dynein light chain 2A" . ., .-..
Assembly members:
Dynein_light_chain_2A,_cytoplasmic, polymer, 101 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Dynein_light_chain_2A,_cytoplasmic: GPLGSMAEVEETLKRLQSQK
GVQGIIVVNTEGIPIKSTMD
NPTTTQYASLMHSFILKARS
TVRDIDPQNDLTFLRIRSKK
NEIMVAPDKDYFLIVIQNPT
E
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 305 |
| 15N chemical shifts | 90 |
| 1H chemical shifts | 649 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | chain A | 1 |
| 2 | chain B | 1 |
Entities:
Entity 1, chain A 101 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLY | SER | MET | ALA | GLU | VAL | GLU | ||||
| 2 | GLU | THR | LEU | LYS | ARG | LEU | GLN | SER | GLN | LYS | ||||
| 3 | GLY | VAL | GLN | GLY | ILE | ILE | VAL | VAL | ASN | THR | ||||
| 4 | GLU | GLY | ILE | PRO | ILE | LYS | SER | THR | MET | ASP | ||||
| 5 | ASN | PRO | THR | THR | THR | GLN | TYR | ALA | SER | LEU | ||||
| 6 | MET | HIS | SER | PHE | ILE | LEU | LYS | ALA | ARG | SER | ||||
| 7 | THR | VAL | ARG | ASP | ILE | ASP | PRO | GLN | ASN | ASP | ||||
| 8 | LEU | THR | PHE | LEU | ARG | ILE | ARG | SER | LYS | LYS | ||||
| 9 | ASN | GLU | ILE | MET | VAL | ALA | PRO | ASP | LYS | ASP | ||||
| 10 | TYR | PHE | LEU | ILE | VAL | ILE | GLN | ASN | PRO | THR | ||||
| 11 | GLU |
Samples:
sample_1: Dynein light chain 2A, cytoplasmic, [U-100% 13C; U-100% 15N], 0.6 mM; phosphate buffer 20 mM; NaCl 0.1 mM; EDTA 20 mM; H2O 95%; D2O 5%
sample_2: Dynein light chain 2A, cytoplasmic, [U-100% 13C; U-100% 15N], 0.6 mM; phosphate buffer 20 mM; NaCl 0.1 mM; EDTA 20 mM; D2O 100%
sample_conditions_1: ionic strength: 40 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D_15N-separated_NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D_13C-separated_NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_2 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRPipe v2.2, Delaglio - data analysis
PIPP v4.3.2, Garrett - data analysis
X-PLOR v3.1f, Brunger - refinement
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAG57523 |
| GB | AAF29126 AAF86646 AAH02481 AAK18712 AAK95342 |
| REF | NP_001177109 NP_001268656 NP_001268657 NP_001268658 NP_054902 |
| SP | Q9NP97 |
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