BMRB Entry 15226
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15226
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Title: High resolution Solid state NMR structure of Kaliotoxin bound to Potassium channel PubMed: 18523586
Deposition date: 2007-04-26 Original release date: 2009-11-20
Authors: Korukottu, Jegannath; Lange, Adam; Vijayan, Vinesh; Schneider, Robert; Pongs, Olaf; Becker, Stefan; Baldus, Marc; Zweckstetter, Markus
Citation: Korukottu, Jegannath; Schneider, Robert; Vijayan, Vinesh; Lange, Adam; Pongs, Olaf; Becker, Stefan; Baldus, Marc; Zweckstetter, Markus. "High-resolution 3D structure determination of kaliotoxin by solid-state NMR spectroscopy." PLoS ONE 3, e2359-. (2008).
Assembly members:
subunit 1, polymer, 38 residues, 4168.079 Da.
Natural source: Common Name: Androctonus mauretanicus Taxonomy ID: 6859 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Androctonus mauretanicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
subunit 1: GVEINVKCSGSPQCLKPCKD
AGMRFGKCMNRKCHCTPK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 100 |
| 15N chemical shifts | 23 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | subunit 1 | 1 |
Entities:
Entity 1, subunit 1 38 residues - 4168.079 Da.
| 1 | GLY | VAL | GLU | ILE | ASN | VAL | LYS | CYS | SER | GLY | ||||
| 2 | SER | PRO | GLN | CYS | LEU | LYS | PRO | CYS | LYS | ASP | ||||
| 3 | ALA | GLY | MET | ARG | PHE | GLY | LYS | CYS | MET | ASN | ||||
| 4 | ARG | LYS | CYS | HIS | CYS | THR | PRO | LYS |
Samples:
sample_1: entity, [U-13C; U-15N], 2 mM
sample_conditions_1: ionic strength: 2 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NHHC | sample_1 | isotropic | sample_conditions_1 |
| CHHC | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 800 MHz