BMRB Entry 15362
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15362
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Title: NMR SOLUTION STRUCTURE OF THE ANTICODON OF E.coli TRNA-VAL3 WITH 1 MODIFICATION (M6A37) PubMed: 18473483
Deposition date: 2007-07-04 Original release date: 2007-07-27
Authors: Vendeix, Franck; Dziergowska, Agnieszka; Gustilo, Estella; Graham, William; Sproat, Brian; Malkiewicz, Andrzej; Agris, Paul
Citation: Vendeix, Franck; Dziergowska, Agnieszka; Gustilo, Estella; Graham, William; Sproat, Brian; Malkiewicz, Andrzej; Agris, Paul. "Anticodon domain modifications contribute order to tRNA for ribosome-mediated codon binding" Biochemistry 47, 6117-6129 (2008).
Assembly members:
RNA_CCUCCCUUAC6MZAGGAGG, polymer, 17 residues, 5402.314 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
RNA_CCUCCCUUAC6MZAGGAGG: CCUCCCUUACXAGGAGG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 120 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ASLval | 1 |
Entities:
Entity 1, ASLval 17 residues - 5402.314 Da.
| 1 | C | C | U | C | C | C | U | U | A | C | ||||
| 2 | 6MZ | A | G | G | A | G | G |
Samples:
(M6A37)-ASL_Val3_H2O: RNA 17mer1.5 – 2 mM
(M6A37)-ASL_Val3_D2O: RNA 17mer1.5 – 2 mM
sample_conditions_1: pH: 6.2; pressure: 1 atm; temperature: 295 K
sample_conditions_2: pH: 6.2; pressure: 1 atm; temperature: 275 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H COSY | (M6A37)-ASL_Val3_D2O | isotropic | sample_conditions_1 |
| 2D DQF-COSY | (M6A37)-ASL_Val3_D2O | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | (M6A37)-ASL_Val3_D2O | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | (M6A37)-ASL_Val3_D2O | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | (M6A37)-ASL_Val3_D2O | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | (M6A37)-ASL_Val3_H2O | isotropic | sample_conditions_2 |
| 2D 1H-31P HETCOR | (M6A37)-ASL_Val3_D2O | isotropic | sample_conditions_1 |
Software:
xwinnmr, Bruker Biospin - collection
VNMR, Varian - collection
NMRDraw v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - data analysis
CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMR spectrometers:
- Bruker Avance 500 MHz
- Varian INOVA 600 MHz