BMRB Entry 15377
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15377
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of human DESR1 PubMed: 18214955
Deposition date: 2007-07-12 Original release date: 2008-03-13
Authors: Wu, Fangming; Wu, Jihui; Shi, Yunyu
Citation: Wu, Fangming; Zhang, J.; Sun, J.; Huang, H.; Ji, P.; Chu, W.; Yu, M.; Yang, F.; Wu, Z.; Wu, J.; Shi, Y.. "Solution structure of human DESR1, a CSL zinc-binding protein." Proteins 71, 514-518 (2008).
Assembly members:
DESR1, polymer, 89 residues, 9512.521 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
DESR1: MAVFHDEVEIEDFQYDEDSE
TYFYPCPCGDNFSITKEDLE
NGEDVATCPSCSLIIKVIYD
KDQFVSGETVPAPSANKELV
KLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 317 |
| 15N chemical shifts | 76 |
| 1H chemical shifts | 535 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DESR1 | 1 |
| 2 | ZN | 2 |
Entities:
Entity 1, DESR1 89 residues - 9512.521 Da.
| 1 | MET | ALA | VAL | PHE | HIS | ASP | GLU | VAL | GLU | ILE | ||||
| 2 | GLU | ASP | PHE | GLN | TYR | ASP | GLU | ASP | SER | GLU | ||||
| 3 | THR | TYR | PHE | TYR | PRO | CYS | PRO | CYS | GLY | ASP | ||||
| 4 | ASN | PHE | SER | ILE | THR | LYS | GLU | ASP | LEU | GLU | ||||
| 5 | ASN | GLY | GLU | ASP | VAL | ALA | THR | CYS | PRO | SER | ||||
| 6 | CYS | SER | LEU | ILE | ILE | LYS | VAL | ILE | TYR | ASP | ||||
| 7 | LYS | ASP | GLN | PHE | VAL | SER | GLY | GLU | THR | VAL | ||||
| 8 | PRO | ALA | PRO | SER | ALA | ASN | LYS | GLU | LEU | VAL | ||||
| 9 | LYS | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Entity 2, ZN - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: entity_1, [U-99% 13C; U-99% 15N], 1.5 mM; TRIS 20 mM; sodium chloride 50 mM; beta-mercaptoethanol 14 mM; H2O 90%; D2O 10%
sample_2: entity_1, [U-99% 13C; U-99% 15N], 1.5 mM; TRIS 20 mM; sodium chloride 50 mM; beta-mercaptoethanol 14 mM; D2O 99.9%
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMRPipe v2.2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY v3, Goddard - data analysis
CSI v1.0, David S. Wishart - data analysis
Molmol v2K.2, Koradi, Billeter and Wuthrich - data analysis
TALOS, Cornilescu, Delaglio and Bax - data analysis
NMR spectrometers:
- Bruker DMX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAE02507 |
| EMBL | CAH92222 |
| GB | AAH10181 AAI68674 ADZ15684 AIC53581 EAW64268 |
| REF | NP_001126299 NP_001247449 NP_996662 XP_002759684 XP_002814073 |
| SP | Q4R312 Q5R7N8 Q96FX2 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts