BMRB Entry 15532
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15532
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Title: Structure of the Wilms Tumor Suppressor Protein Zinc Finger Domain Bound to DNA PubMed: 17716689
Deposition date: 2007-10-18 Original release date: 2008-02-14
Authors: Stoll, Raphael; Lee, Brian; Debler, Erik; Laity, John; Wilson, Ian; Dyson, Helen; Wright, Peter
Citation: Stoll, Raphael; Lee, Brian; Debler, Erik; Laity, John; Wilson, Ian; Dyson, Helen; Wright, Peter. "Structure of the Wilms' Tumor Suppressor Protein Zinc Finger Domain Bound to DNA" J. Mol. Biol. 372, 1227-1245 (2007).
Assembly members:
wt1-17mer, polymer, 119 residues, 14494.849 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
wt1-17mer: ASEKRPFMCAYPGCNKRYFK
LSHLQMHSRKHTGEKPYQCD
FKDCERRFSRSDQLKRHQRR
HTGVKPFQCKTCQRKFSRSD
HLKTHTRTHTGEKPFSCRWP
SCQKKFARSDELVRHHNMH
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 110 |
| 1H chemical shifts | 110 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | wt1-17mer | 1 |
| 2 | zn1 | 2 |
| 3 | zn2 | 2 |
| 4 | zn3 | 2 |
| 5 | zn4 | 2 |
Entities:
Entity 1, wt1-17mer 119 residues - 14494.849 Da.
| 1 | ALA | SER | GLU | LYS | ARG | PRO | PHE | MET | CYS | ALA | ||||
| 2 | TYR | PRO | GLY | CYS | ASN | LYS | ARG | TYR | PHE | LYS | ||||
| 3 | LEU | SER | HIS | LEU | GLN | MET | HIS | SER | ARG | LYS | ||||
| 4 | HIS | THR | GLY | GLU | LYS | PRO | TYR | GLN | CYS | ASP | ||||
| 5 | PHE | LYS | ASP | CYS | GLU | ARG | ARG | PHE | SER | ARG | ||||
| 6 | SER | ASP | GLN | LEU | LYS | ARG | HIS | GLN | ARG | ARG | ||||
| 7 | HIS | THR | GLY | VAL | LYS | PRO | PHE | GLN | CYS | LYS | ||||
| 8 | THR | CYS | GLN | ARG | LYS | PHE | SER | ARG | SER | ASP | ||||
| 9 | HIS | LEU | LYS | THR | HIS | THR | ARG | THR | HIS | THR | ||||
| 10 | GLY | GLU | LYS | PRO | PHE | SER | CYS | ARG | TRP | PRO | ||||
| 11 | SER | CYS | GLN | LYS | LYS | PHE | ALA | ARG | SER | ASP | ||||
| 12 | GLU | LEU | VAL | ARG | HIS | HIS | ASN | MET | HIS |
Entity 2, zn1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: wt1, [U-100% 13C; U-100% 15N], 0.6 mM; Tris/HCl 10 mM; KCl 20 mM; ZnSO4 5 uM; NaN3 2 mM
sample_conditions_1: ionic strength: 20 mM; pH: 6.7; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| IPAP | sample_1 | anisotropic | sample_conditions_1 |
Software:
AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Koll - structure solution
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 800 MHz
- Bruker Avance 900 MHz
Related Database Links:
| BMRB | 15533 4707 4708 4709 4710 |
| PDB | |
| DBJ | BAA11522 BAA28147 BAA94793 BAF84425 |
| EMBL | CAA35956 CAA43819 CDG23662 |
| GB | AAA36810 AAA61299 AAB33443 AAB53152 AAH32861 |
| PRF | 1604420A |
| REF | NP_000369 NP_001001264 NP_001079057 NP_001079336 NP_001135625 |
| SP | B5DE03 O62651 P19544 P79958 |
| TPG | DAA21902 |
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