BMRB Entry 15609
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15609
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Title: solution structure of 50S ribosomal protein L28 from Thermotoga maritima: Northeast Structural Genomics consortium target VR97
Deposition date: 2007-12-28 Original release date: 2008-01-25
Authors: Wu, Yibing; Singarapu, Kiran Kumar; Guido, Valerie; Yee, Adelinda; Sukumaran, Dinesh; Arrowsmith, Cheryl; Szyperski, Thomas
Citation: Wu, Yibing; Singarapu, Kiran Kumar; Guido, Valerie; Yee, Adelinda; Sukumaran, Dinesh; Arrowsmith, Cheryl; Szyperski, Thomas. "solution structure of 50S ribosomal protein L28 from Thermotoga maritima: Northeast Structural Genomics consortium target VR97" . ., .-..
Assembly members:
ribosomal protein L28, polymer, 77 residues, 8612.257 Da.
Natural source: Common Name: Thermotoga maritima Taxonomy ID: 2336 Superkingdom: Bacteria Kingdom: not available Genus/species: Thermotoga maritima
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ribosomal protein L28: QGHMIMAKRCEVCGKAPRSG
NTVSHSDKKSERWFRPNLQK
VRVVLPDGTIKRMRVCTSCL
KSGKVKKYVGQVSEVGS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 210 |
| 15N chemical shifts | 61 |
| 1H chemical shifts | 440 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ribosomal protein L28 | 1 |
Entities:
Entity 1, ribosomal protein L28 77 residues - 8612.257 Da.
| 1 | GLN | GLY | HIS | MET | ILE | MET | ALA | LYS | ARG | CYS | ||||
| 2 | GLU | VAL | CYS | GLY | LYS | ALA | PRO | ARG | SER | GLY | ||||
| 3 | ASN | THR | VAL | SER | HIS | SER | ASP | LYS | LYS | SER | ||||
| 4 | GLU | ARG | TRP | PHE | ARG | PRO | ASN | LEU | GLN | LYS | ||||
| 5 | VAL | ARG | VAL | VAL | LEU | PRO | ASP | GLY | THR | ILE | ||||
| 6 | LYS | ARG | MET | ARG | VAL | CYS | THR | SER | CYS | LEU | ||||
| 7 | LYS | SER | GLY | LYS | VAL | LYS | LYS | TYR | VAL | GLY | ||||
| 8 | GLN | VAL | SER | GLU | VAL | GLY | SER |
Samples:
sample_1: entity 1.0 mM; NaCl 300 mM; D2O, [U-100% 2H], 10%; H2O 90%
sample_conditions_1: ionic strength: 0.3 M; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 4,3D, GFT HNNCABCA | sample_1 | isotropic | sample_conditions_1 |
| 4,3D, GFT CABCACONNH | sample_1 | isotropic | sample_conditions_1 |
| 4,3D, GFT HCCH COSY | sample_1 | isotropic | sample_conditions_1 |
| 3D, 15N-13C RESOLVEDSIMULTANIOUS NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment
XEASY, Bartels et al. - peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
AutoStruct, Huang, Tejero, Powers and Montelione - structure solution
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Varian INOVA 750 MHz
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| GB | AAD35344 ABQ46689 ACB09046 ADA66976 AGL49179 |
| REF | NP_228069 WP_010865082 WP_015646089 |
| SP | B1L9P8 Q9WY96 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts