BMRB Entry 15622
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15622
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Title: Solution structure of an antilipopolysaccharide factor from shrimp PubMed: 18466970
Deposition date: 2008-01-09 Original release date: 2008-09-19
Authors: Yang, Yinshan; Boze, Helene; Chemardin, Patrick; Padilla, Andre; Moulin, Guy; Tassanakajon, Anchalaee; Pugniere, Martine; Roquet, Francoise; Gueguen, Yannick; Bachere, Evelyne; Aumelas, Andre
Citation: Somboonwiwat, Kunlaya; Bachere, Evelyne; Rimphanitchayakit, Vichien; Tassanakajon, Anchalaee. "Localization of anti-lipopolysaccharide factor (ALFPm3) in tissues of the black tiger shrimp, Penaeus monodon, and characterization of its binding properties." Dev. Comp. Immunol. 32, 1170-1176 (2008).
Assembly members:
antilipopolysaccharide_factor, polymer, 102 residues, 11534.318 Da.
Natural source: Common Name: black tiger shrimp Taxonomy ID: 6687 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Penaeus monodon
Experimental source: Production method: recombinant technology Host organism: Pichia pastoris
Entity Sequences (FASTA):
antilipopolysaccharide_factor: EAYVQGWEAVAAAVASKIVG
LWRNEKTELLGHECKFTVKP
YLKRFQVYYKGRMWCPGWTA
IRGEASTRSQSGVAGKTAKD
FVRKAFQKGLISQQEANQWL
SS
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 90 |
| 1H chemical shifts | 619 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | antilipopolysaccharide factor | 1 |
Entities:
Entity 1, antilipopolysaccharide factor 102 residues - 11534.318 Da.
| 1 | GLU | ALA | TYR | VAL | GLN | GLY | TRP | GLU | ALA | VAL | ||||
| 2 | ALA | ALA | ALA | VAL | ALA | SER | LYS | ILE | VAL | GLY | ||||
| 3 | LEU | TRP | ARG | ASN | GLU | LYS | THR | GLU | LEU | LEU | ||||
| 4 | GLY | HIS | GLU | CYS | LYS | PHE | THR | VAL | LYS | PRO | ||||
| 5 | TYR | LEU | LYS | ARG | PHE | GLN | VAL | TYR | TYR | LYS | ||||
| 6 | GLY | ARG | MET | TRP | CYS | PRO | GLY | TRP | THR | ALA | ||||
| 7 | ILE | ARG | GLY | GLU | ALA | SER | THR | ARG | SER | GLN | ||||
| 8 | SER | GLY | VAL | ALA | GLY | LYS | THR | ALA | LYS | ASP | ||||
| 9 | PHE | VAL | ARG | LYS | ALA | PHE | GLN | LYS | GLY | LEU | ||||
| 10 | ILE | SER | GLN | GLN | GLU | ALA | ASN | GLN | TRP | LEU | ||||
| 11 | SER | SER |
Samples:
sample_1: ALF 1 mM
sample_2: ALF, [U-99% 15N], 1 mM
sample_conditions_1: ionic strength: 0 M; pH: 6.9; pressure: 1 atm; temperature: 305 K
sample_conditions_2: ionic strength: 0 M; pH: 6.9; pressure: 1 atm; temperature: 305 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_2 |
| 3D 1H-15N HSQC-NOESY | sample_2 | isotropic | sample_conditions_2 |
Software:
xwinnmr, Bruker Biospin - collection
GIFA, Delsuc - processing
X-PLOR, Brunger - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
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