BMRB Entry 15701
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15701
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Title: solution struture of human C-terminal domain of pirh2/Northeast Structural Genomics Consortium (NESG) target HT2C PubMed: 19043414
Deposition date: 2008-03-31 Original release date: 2008-04-07
Authors: Lemak, Alexander; Sheng, Yi; Karra, Murthy; Srisailam, Sampath; Laister, Rob; Duan, Shili; Arrowsmith, Cheryl
Citation: Sheng, Yi; Laister, Rob; Lemak, Alexander; Wu, Bin; Tai, Elizabeth; Duan, Shili; Lukin, Jonathan; Sunnerhagen, Maria; Srisailam, Sampath; Karra, Murthy; Benchimol, Sam; Arrowsmith, Cheryl. "Molecular basis of Pirh2-mediated p53 ubiquitylation" Nat. Struct. Mol. Biol. 15, 1334-1342 (2008).
Assembly members:
human_C-termal_doamin_of_pirh2, polymer, 79 residues, 5240.008 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
human_C-termal_doamin_of_pirh2: GSHMMHSALDMTRYWRQLDD
EVAQTPMPSEYQNMTVDILC
NDCNGRSTVQFHILGMKCKI
CESYNTAQAGGRRISLDQQ
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 308 |
| 15N chemical shifts | 72 |
| 1H chemical shifts | 497 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, entity_1 79 residues - 5240.008 Da.
| 1 | GLY | SER | HIS | MET | MET | HIS | SER | ALA | LEU | ASP | ||||
| 2 | MET | THR | ARG | TYR | TRP | ARG | GLN | LEU | ASP | ASP | ||||
| 3 | GLU | VAL | ALA | GLN | THR | PRO | MET | PRO | SER | GLU | ||||
| 4 | TYR | GLN | ASN | MET | THR | VAL | ASP | ILE | LEU | CYS | ||||
| 5 | ASN | ASP | CYS | ASN | GLY | ARG | SER | THR | VAL | GLN | ||||
| 6 | PHE | HIS | ILE | LEU | GLY | MET | LYS | CYS | LYS | ILE | ||||
| 7 | CYS | GLU | SER | TYR | ASN | THR | ALA | GLN | ALA | GLY | ||||
| 8 | GLY | ARG | ARG | ILE | SER | LEU | ASP | GLN | GLN |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_2: human C-termal doamin of pirh2, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 50 mM; potassium chloride 150 mM; ZnCl2 10 uM; DTT 0.8 mM
sample_1: human C-termal doamin of pirh2, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 50 mM; potassium chloride 150 mM; ZnCl2 10 uM; DTT 0.8 mM
sample_conditions_1: ionic strength: 150 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D (H)CCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C-Aromatic NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - peak picking
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
AutoStruct, Huang, Tejero, Powers and Montelione - Validation
TALOS, Cornilescu, Delaglio and Bax - data analysis
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
- Varian INOVA 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAD92309 BAG52375 BAI46763 |
| GB | AAH15464 AAH47393 AAK96896 AAL09356 AAL76101 |
| REF | NP_001009922 NP_001090959 NP_001248474 NP_001265465 NP_001265466 |
| SP | Q96PM5 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts