BMRB Entry 15747
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15747
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Title: Structural and Functional Characterization of TM IX of the NHE1 Isoform of the Na+/H+ Exchanger PubMed: 18508767
Deposition date: 2008-05-01 Original release date: 2008-05-29
Authors: Reddy, Tyler; Ding, J.; Li, X.; Sykes, Brian; Fliegel, Larry; Rainey, Jan
Citation: Reddy, Tyler; Ding, J.; Li, X.; Sykes, Brian; Rainey, Jan; Fliegel, Larry. "Structural and Functional Characterization of TM IX of the NHE1 Isoform of the Na+/H+ Exchanger" J. Biol. Chem. 283, 22018-22030 (2008).
Assembly members:
TMIX_peptide, polymer, 33 residues, 3444.226 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
TMIX_peptide: XKSYMAYLSAELFHLSGIMA
LIASGVVMRPKKX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 235 |
| 13C chemical shifts | 97 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TMIX_peptide | 1 |
Entities:
Entity 1, TMIX_peptide 33 residues - 3444.226 Da.
| 1 | ACE | LYS | SER | TYR | MET | ALA | TYR | LEU | SER | ALA | ||||
| 2 | GLU | LEU | PHE | HIS | LEU | SER | GLY | ILE | MET | ALA | ||||
| 3 | LEU | ILE | ALA | SER | GLY | VAL | VAL | MET | ARG | PRO | ||||
| 4 | LYS | LYS | NH2 |
Samples:
sample_TMIX_peptide: TMIX peptide 0.9 ± 0.1 mM; DPC-d38 75 mM; DSS 0.25 mM; H2O 95%; D2O 5%
sample_conditions_1: temperature: 303.15 K; pH: 5.05; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC | sample_TMIX_peptide | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_TMIX_peptide | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_TMIX_peptide | isotropic | sample_conditions_1 |
Software:
X-PLOR_NIH v2.18, Schwieters, Kuszewski, Tjandra and Clore - structure solution
SPARKY v3.112, Goddard - peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 800 MHz