BMRB Entry 15777
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15777
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution Structure of IG-Like Domain 23 from Human Filamin A PubMed: 19293932
Deposition date: 2008-05-16 Original release date: 2009-05-22
Authors: Heikkinen, Outi; Kupiainen, Olga; Ylanne, Jari; Kilpelainen, Ilkka
Citation: Nakamura, Fumihiko; Heikkinen, Outi; Pentikainen, Olli; Osborn, Teresia; Kasza, Karen; Weitz, David; Kupiainen, Olga; Permi, Perttu; Kilpelainen, Ilkka; Ylanne, Jari; Hartwig, John; Stossel, Thomas. "Molecular basis of filamin A-FilGAP interaction and its impairment in congenital disorders associated with filamin A mutations" PLoS ONE 4, 4928-4928 (2009).
Assembly members:
FLNA23, polymer, 99 residues, 9989.371 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
FLNA23: GAMGDPGLVSAYGAGLEGGV
TGNPAEFVVNTSNAGAGALS
VTIDGPSKVKMDCQECPEGY
RVTYTPMAPGSYLISIKYGG
PYHIGGSPFKAKVTGPRLV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 374 |
| 15N chemical shifts | 92 |
| 1H chemical shifts | 627 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FLNA23 | 1 |
Entities:
Entity 1, FLNA23 99 residues - 9989.371 Da.
| 1 | GLY | ALA | MET | GLY | ASP | PRO | GLY | LEU | VAL | SER | ||||
| 2 | ALA | TYR | GLY | ALA | GLY | LEU | GLU | GLY | GLY | VAL | ||||
| 3 | THR | GLY | ASN | PRO | ALA | GLU | PHE | VAL | VAL | ASN | ||||
| 4 | THR | SER | ASN | ALA | GLY | ALA | GLY | ALA | LEU | SER | ||||
| 5 | VAL | THR | ILE | ASP | GLY | PRO | SER | LYS | VAL | LYS | ||||
| 6 | MET | ASP | CYS | GLN | GLU | CYS | PRO | GLU | GLY | TYR | ||||
| 7 | ARG | VAL | THR | TYR | THR | PRO | MET | ALA | PRO | GLY | ||||
| 8 | SER | TYR | LEU | ILE | SER | ILE | LYS | TYR | GLY | GLY | ||||
| 9 | PRO | TYR | HIS | ILE | GLY | GLY | SER | PRO | PHE | LYS | ||||
| 10 | ALA | LYS | VAL | THR | GLY | PRO | ARG | LEU | VAL |
Samples:
sample_1: FLNA23, [U-13C; U-15N], 1 ± 0.2 mM; Sodium phosphate 20 mM; Sodium chloride 150 mM; DTT 1 mM; Sodium azide 2 mM
sample_conditions_1: ionic strength: 150 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
| iHNCA | sample_1 | isotropic | sample_conditions_1 |
| iHNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMR v6.1C, Varian - collection, data analysis, processing
SPARKY v3.110, Goddard - chemical shift assignment, data analysis, peak picking
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
AMBER v8.0, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts