BMRB Entry 15811
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15811
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Title: Solution structure of 30S ribosomal protein S27A from Thermoplasma acidophilum
Deposition date: 2008-06-20 Original release date: 2009-04-23
Authors: Wu, Bin; Yee, Adelinda; Fares, Christophe; Lemak, Alexander; Semest, Anthony; Arrowsmith, Cheryl
Citation: Wu, Bin; Yee, Adelinda; Fares, Christophe; Lemak, Alexander; Semest, Anthony; Arrowsmith, Cheryl. "Solution structure of 30S ribosomal protein S27A from Thermoplasma acidophilum/Northeast Structural Genomics Consortium Target TaT88/Ontario Center for Structural Proteomics target ta1093" Not known ., .-..
Assembly members:
30S_ribosomal_protein_S27A, polymer, 55 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Thermoplasma acidophila Taxonomy ID: 2303 Superkingdom: Archaea Kingdom: not available Genus/species: Thermoplasma acidophilum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
30S_ribosomal_protein_S27A: MQKRELYEIADGKLVRKHRF
CPRCGPGVFLAEHADRYSCG
RCGYTEFKKAKKSKS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 224 |
| 15N chemical shifts | 51 |
| 1H chemical shifts | 353 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 30S_ribosomal | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, 30S_ribosomal 55 residues - Formula weight is not available
| 1 | MET | GLN | LYS | ARG | GLU | LEU | TYR | GLU | ILE | ALA | ||||
| 2 | ASP | GLY | LYS | LEU | VAL | ARG | LYS | HIS | ARG | PHE | ||||
| 3 | CYS | PRO | ARG | CYS | GLY | PRO | GLY | VAL | PHE | LEU | ||||
| 4 | ALA | GLU | HIS | ALA | ASP | ARG | TYR | SER | CYS | GLY | ||||
| 5 | ARG | CYS | GLY | TYR | THR | GLU | PHE | LYS | LYS | ALA | ||||
| 6 | LYS | LYS | SER | LYS | SER |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_2: 30S ribosomal protein S27A, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; ZnSO4 10 uM; DTT, [U-100% 2H], 10 mM; sodium azide 0.01%; benzamidine 10 mM; D2O, [U-100% 2H], 100%
sample_1: 30S ribosomal protein S27A, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; ZnSO4 10 uM; DTT, [U-100% 2H], 10 mM; sodium azide 0.01%; benzamidine 10 mM; H2O 90%; D2O, [U-100% 2H], 10%
sample_conditions_1: ionic strength: 300 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY v3.95, Goddard - data analysis, peak picking
AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
CNS v1.0, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
AutoStruct, Huang, Tejero, Powers and Montelione - NMR structure quality assessment
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts