BMRB Entry 15812
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15812
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Title: NMR Structure of FeoA-like protein from Clostridium acetobutylicum: Northeast Structural Genomics Consortium Target CaR178
Deposition date: 2008-06-20 Original release date: 2008-08-08
Authors: SINGARAPU, KIRAN KUMAR; WU, YIBING; HUA, JIA; SUKUMARAN, DINESH; Zhao, Li; Jiang, Mei; Foote, Erica; Xiao, Rong; Nair, Rajesh; Baran, Michael; G.V.T, Swapna; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; SZYPERSKI, THOMAS
Citation: SINGARAPU, KIRAN KUMAR; WU, YIBING; HUA, JIA; SUKUMARAN, DINESH; Zhao, Li; Jiang, Mei; Foote, Erica; Xiao, Rong; Nair, Rajesh; Baran, Michael; G.V.T, Swapna; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; SZYPERSKI, THOMAS. "NMR Structure of FeoA-like protein from Clostridium acetobutylicum: Northeast Structural Genomics Consortium Target CaR178" Not known ., .-..
Assembly members:
FeoA-like protein, polymer, 86 residues, 9724.640 Da.
Natural source: Common Name: Clostridium acetobutylicum Taxonomy ID: 1488 Superkingdom: Bacteria Kingdom: not available Genus/species: Clostridium acetobutylicum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
FeoA-like protein: MTKGIGLNEVEIKSKVKVIG
IVPESKVRRKIMDMGIVRGT
EIYIEGKAPMGDPIALRLRG
YSLSLRKSEAKDILVEVLLE
HHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 368 |
| 15N chemical shifts | 78 |
| 1H chemical shifts | 619 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FeoA-like protein | 1 |
Entities:
Entity 1, FeoA-like protein 86 residues - 9724.640 Da.
| 1 | MET | THR | LYS | GLY | ILE | GLY | LEU | ASN | GLU | VAL | ||||
| 2 | GLU | ILE | LYS | SER | LYS | VAL | LYS | VAL | ILE | GLY | ||||
| 3 | ILE | VAL | PRO | GLU | SER | LYS | VAL | ARG | ARG | LYS | ||||
| 4 | ILE | MET | ASP | MET | GLY | ILE | VAL | ARG | GLY | THR | ||||
| 5 | GLU | ILE | TYR | ILE | GLU | GLY | LYS | ALA | PRO | MET | ||||
| 6 | GLY | ASP | PRO | ILE | ALA | LEU | ARG | LEU | ARG | GLY | ||||
| 7 | TYR | SER | LEU | SER | LEU | ARG | LYS | SER | GLU | ALA | ||||
| 8 | LYS | ASP | ILE | LEU | VAL | GLU | VAL | LEU | LEU | GLU | ||||
| 9 | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: entity, [U-100% 13C; U-100% 15N], 0.91 mM
sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 4.3D GFT HNNCABCA | sample_1 | isotropic | sample_conditions_1 |
| 4,3D GFT CABCACONH | sample_1 | isotropic | sample_conditions_1 |
| 4,3D GFT HABCABCONH | sample_1 | isotropic | sample_conditions_1 |
| 4,3D GFT HCCH COSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 15N, 13C resolved NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
XEASY, Bartels et al. - data analysis
TALOS, Cornilescu, Delaglio and Bax - data analysis
CYANA, Guntert, Mumenthaler and Wuthrich - data analysis
AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment
AutoStruct, Huang, Tejero, Powers and Montelione - data analysis
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
Molmol, Koradi, Billeter and Wuthrich - structure solution
NMR spectrometers:
- Varian INOVA 750 MHz
Related Database Links:
| PDB | |
| GB | AAK79005 ADZ20080 AEI31564 KHD35643 |
| REF | NP_347665 WP_010964347 WP_034584547 |
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