BMRB Entry 15880
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15880
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Structure of Rab11-FIP2 C-terminal Coiled-coil Domain PubMed: 19119858
Deposition date: 2008-07-18 Original release date: 2009-02-13
Authors: Wei, Jie; Liu, Yuqi; Baleja, James
Citation: Wei, Jie; Liu, Yuqi; Bose, Kakoli; Henry, Gillian; Baleja, Jim. "Disorder and structure in the Rab11 binding domain of Rab11 family interacting protein 2." Biochemistry 48, 549-557 (2009).
Assembly members:
Rab11-FIP2, polymer, 41 residues, 5004.829 Da.
Natural source: Common Name: Human Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Human sapien
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Rab11-FIP2: GSLTYEEVLQELVKHKELLR
RKDTHIRELEDYIDNLLVRV
M
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 60 |
| 15N chemical shifts | 35 |
| 1H chemical shifts | 299 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 1 |
Entities:
Entity 1, entity_1 41 residues - 5004.829 Da.
| 1 | GLY | SER | LEU | THR | TYR | GLU | GLU | VAL | LEU | GLN | ||||
| 2 | GLU | LEU | VAL | LYS | HIS | LYS | GLU | LEU | LEU | ARG | ||||
| 3 | ARG | LYS | ASP | THR | HIS | ILE | ARG | GLU | LEU | GLU | ||||
| 4 | ASP | TYR | ILE | ASP | ASN | LEU | LEU | VAL | ARG | VAL | ||||
| 5 | MET |
Samples:
sample_1: entity 1 mM; DSS 20 uM; sodium phosphate 20 mM; D2O, [U-100% 2H], 100%
sample_2: entity, [U-100% 15N], 1.5 mM; DSS 20 uM; sodium phosphate 20 mM; H2O 90%; D2O, [U-100% 2H], 10%
sample_3: entity, [U-100% 13C; U-100% 15N], 0.8 mM; DSS 20 uM; sodium phosphate 20 mM; H2O 90%; D2O, [U-100% 2H], 10%
sample_conditions_1: ionic strength: 0.08 M; pH: 7.2; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_3 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_3 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_3 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_3 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_3 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_3 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
Software:
xwinnmr v3.5, Bruker Biospin - collection
TOPSPIN v1.3, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA76785 BAE32923 BAG09909 |
| GB | AAH75073 AAH75074 AAH89010 AAI39381 AAI39420 |
| REF | NP_001028344 NP_001100917 NP_001157839 NP_001179700 NP_055719 |
| SP | G3XA57 Q7L804 |
| TPG | DAA14748 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts