BMRB Entry 15919
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15919
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Title: NMR structure of a complex formed by the C-terminal domain of human RAP74 and a phosphorylated peptide from the central domain of the FCP1 PubMed: 19215094
Deposition date: 2008-08-13 Original release date: 2009-02-16
Authors: Yang, Ao; Abbott, Karen; Desjardins, Alexandre; Di Lello, Paola; Omichinski, James; Legault, Pascale
Citation: Yang, Ao; Abbott, Karen; Desjardins, Alexandre; Di Lello, Paola; Omichinski, James; Legault, Pascale. "NMR structure of a complex formed by the carboxyl-terminal domain of human RAP74 and a phosphorylated peptide from the central domain of the FCP1 Phosphatase" Biochemistry 48, 1964-1974 (2009).
Assembly members:
centFCP1-T584PO4, polymer, 19 residues, 2398.493 Da.
cterRAP74, polymer, 82 residues, 7906.366 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
centFCP1-T584PO4: EDXDEDDHLIYLEEILVRV
cterRAP74: STPQPPSGKTTPNSGDVQVT
EDAVRRYLTRKPMTTKDLLK
KFQTKKTGLSSEQTVNVLAQ
ILKRLNPERKMINDKMHFSL
KE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 345 |
| 15N chemical shifts | 85 |
| 1H chemical shifts | 589 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | centFCP1-T584PO4 | 1 |
| 2 | cterRAP74 | 2 |
Entities:
Entity 1, centFCP1-T584PO4 19 residues - 2398.493 Da.
| 1 | GLU | ASP | TPO | ASP | GLU | ASP | ASP | HIS | LEU | ILE | ||||
| 2 | TYR | LEU | GLU | GLU | ILE | LEU | VAL | ARG | VAL |
Entity 2, cterRAP74 82 residues - 7906.366 Da.
| 1 | SER | THR | PRO | GLN | PRO | PRO | SER | GLY | LYS | THR | ||||
| 2 | THR | PRO | ASN | SER | GLY | ASP | VAL | GLN | VAL | THR | ||||
| 3 | GLU | ASP | ALA | VAL | ARG | ARG | TYR | LEU | THR | ARG | ||||
| 4 | LYS | PRO | MET | THR | THR | LYS | ASP | LEU | LEU | LYS | ||||
| 5 | LYS | PHE | GLN | THR | LYS | LYS | THR | GLY | LEU | SER | ||||
| 6 | SER | GLU | GLN | THR | VAL | ASN | VAL | LEU | ALA | GLN | ||||
| 7 | ILE | LEU | LYS | ARG | LEU | ASN | PRO | GLU | ARG | LYS | ||||
| 8 | MET | ILE | ASN | ASP | LYS | MET | HIS | PHE | SER | LEU | ||||
| 9 | LYS | GLU |
Samples:
15N-labeled: cterRAP74, [U-98% 15N], 1 mM; centFCP1-T584PO4 1 mM; H2O 90%; D2O, [U-100% 2H], 10%; sodium phosphate 10-40 mM; EDTA 0.25 mM
13C_15N-labeled-H2O: cterRAP74, [U-98% 13C; U-98% 15N], 1 mM; centFCP1-T584PO4 1 mM; H2O 90%; D2O, [U-100% 2H], 10%; sodium phosphate 10-40 mM; EDTA 0.25 mM
13C_15N-labeled-D2O: cterRAP74, [U-98% 13C; U-98% 15N], 1 mM; centFCP1-T584PO4 1 mM; D2O, [U-100% 2H], 100%; sodium phosphate 10-40 mM; EDTA 0.25 mM
sample_conditions_1: ionic strength: 25 mM; pH: 6.5; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | 15N-labeled | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | 13C_15N-labeled-H2O | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | 13C_15N-labeled-D2O | isotropic | sample_conditions_1 |
| 3D HNHA | 15N-labeled | isotropic | sample_conditions_1 |
| 3D HNCACB | 13C_15N-labeled-H2O | isotropic | sample_conditions_1 |
| 3D HNCO | 13C_15N-labeled-H2O | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | 13C_15N-labeled-H2O | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | 13C_15N-labeled-D2O | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | 13C_15N-labeled-D2O | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | 15N-labeled | isotropic | sample_conditions_1 |
| 3D CCCTOCSY-NHH | 13C_15N-labeled-H2O | isotropic | sample_conditions_1 |
| 3D HCCTOCSY-NHH | 13C_15N-labeled-H2O | isotropic | sample_conditions_1 |
| 3D 13C-edited HMQC-NOESY | 13C_15N-labeled-D2O | isotropic | sample_conditions_1 |
| HBCBCGCDHD_ARO | 13C_15N-labeled-D2O | isotropic | sample_conditions_1 |
| HBCBCGCDCEHE_ARO | 13C_15N-labeled-D2O | isotropic | sample_conditions_1 |
| 2D isotope filtered 1H-1H TOCSY | 13C_15N-labeled-D2O | isotropic | sample_conditions_1 |
| 2D isotope filtered 1H-1H NOESY | 13C_15N-labeled-D2O | isotropic | sample_conditions_1 |
| 2D isotope filtered 1H-1H TOCSY | 13C_15N-labeled-H2O | isotropic | sample_conditions_1 |
| 2D isotope filtered 1H-1H NOESY | 13C_15N-labeled-H2O | isotropic | sample_conditions_1 |
| 2D 13C F1-filtered, F2-edited NOESY | 13C_15N-labeled-H2O | isotropic | sample_conditions_1 |
| 3D 15N/13C F1-filtered, F3-edited NOESY | 13C_15N-labeled-D2O | isotropic | sample_conditions_1 |
Software:
NMRPipe v2.5, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView v5.2.2_01, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking
NMRDraw v2.5, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing
CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMR spectrometers:
- Varian Unity 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAG11094 BAG37667 BAG56676 BAG63544 BAK63776 |
| EMBL | CAA45404 CAA45408 CAG33079 |
| GB | AAH00120 AAH13007 AAP35761 AAP36816 AAX32213 |
| PRF | 1804352A 1804353A |
| REF | NP_001267379 NP_002087 XP_003281617 XP_003461160 XP_003819382 |
| SP | P35269 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts