BMRB Entry 15944

Assembly:

Entities:

Entity 1, TAZ2 90 residues - 9963.838 Da.

1-90 from p300(1723-1812)

Entity 2, TAD(1-39) 39 residues - 4351.815 Da.

1-39

Samples:

p53cpx_3: TAZ2 1.1-1.2 mM; TAD(1-39), [U-100% 15N], 1.0-1.1 mM; MES 50 mM; NaCl 200 mM; ZnCl 0.1 mM; DTT 1 mM

TAZ2cpx_1: TAZ2, [U-100% 15N], 1.0-1.2 mM; TAD(1-39) 1.1-1.3 mM; MES 50 mM; NaCl 200 mM; ZnCl 0.1 mM; DTT 1 mM

TAZ2cpx_2: TAZ2, [U-100% 13C; U-100% 15N], 1.0-1.2 mM; TAD(1-39) 1.1-1.3 mM; MES 50 mM; NaCl 200 mM; ZnCl 0.1 mM; DTT 1 mM

TAZ2cpx_4: TAZ2 1.0-1.2 mM; TAD(1-39) 1.1-1.3 mM; MES 50 mM; NaCl 200 mM; ZnCl 0.1 mM; DTT 1 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6.3; pressure: 1 atm; temperature: 308 K

Experiments:

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRPipe vupdated, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRView vupdated, Johnson, One Moon Scientific - chemical shift assignment

X-PLOR NIH v2.19, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

TALOS vupdated, Cornilescu, Delaglio and Bax - structure solution

ProcheckNMR vupdated, Laskowski and MacArthur - structure validation

MolProbity v3.15, Richardson - structure validation

InsightII vupdated, Accelrys Software Inc. - figure preparation

SYBYL v8.0, Tripos - structure solution

NMR spectrometers:

• Bruker Avance 500 MHz
• Bruker DRX 700 MHz