BMRB Entry 15948
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15948
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: 1H, 13C, and 15N Chemical Shift Assignments for the RING finger of the E3 ubiquitin ligase Arkadia (RNF111) PubMed: 19032943
Deposition date: 2008-09-10 Original release date: 2009-01-16
Authors: Kandias, Nikolaos; Chasapis, Christos; Episkopou, Vasso; Bentrop, Detlef; Spyroulias, Georgios
Citation: Kandias, Nikolaos; Chasapis, Christos; Bentrop, Detlef; Episkopou, Vasso; Spyroulias, Georgios. "High yield expression and NMR characterization of Arkadia E3 ubiquitin ligase RING-H2 finger domain" Biochem. Biophys. Res. Commun. 378, 498-502 (2009).
Assembly members:
E3_ubiquitin_ligase_Arkadia, polymer, 69 residues, 7951.0 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
E3_ubiquitin_ligase_Arkadia: MKQDGEEGTEEDTEEKCTIC
LSILEEGEDVRRLPCMHLFH
QVCVDQWLITNKKCPICRVD
IEAQLPSES
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 290 |
| 15N chemical shifts | 71 |
| 1H chemical shifts | 467 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RING Finger | 1 |
| 2 | Zinc ion, 1 | 2 |
| 3 | Zinc ion, 2 | 2 |
Entities:
Entity 1, RING Finger 69 residues - 7951.0 Da.
| 1 | MET | LYS | GLN | ASP | GLY | GLU | GLU | GLY | THR | GLU | ||||
| 2 | GLU | ASP | THR | GLU | GLU | LYS | CYS | THR | ILE | CYS | ||||
| 3 | LEU | SER | ILE | LEU | GLU | GLU | GLY | GLU | ASP | VAL | ||||
| 4 | ARG | ARG | LEU | PRO | CYS | MET | HIS | LEU | PHE | HIS | ||||
| 5 | GLN | VAL | CYS | VAL | ASP | GLN | TRP | LEU | ILE | THR | ||||
| 6 | ASN | LYS | LYS | CYS | PRO | ILE | CYS | ARG | VAL | ASP | ||||
| 7 | ILE | GLU | ALA | GLN | LEU | PRO | SER | GLU | SER |
Entity 2, Zinc ion, 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: E3 ubiquitin ligase Arkadia, [U-98% 13C; U-98% 15N], 1.0 ± 0.2 mM; Pi 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.9; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D (HB)CB(CGCD)HDarom | sample_1 | isotropic | sample_conditions_1 |
| 2D 2J-edited 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA v1.8.4, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAC04531 BAC29394 BAC33245 BAD18471 BAD18633 |
| EMBL | CAB75669 CAD98031 CAH93440 |
| GB | AAH10369 AAH20984 AAH54842 AAH60862 AAH69835 |
| REF | NP_001095709 NP_001100306 NP_001124565 NP_001186680 NP_001257457 |
| SP | Q5R476 Q6ZNA4 Q99ML9 |
| TPG | DAA25343 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts