BMRB Entry 15951
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15951
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Title: HCV p7 tm2 PubMed: 20667830
Deposition date: 2008-09-12 Original release date: 2009-05-29
Authors: Montserret, Roland; Penin, Francois
Citation: Montserret, Roland; Saint, Nathalie; Vanbelle, Christophe; Salvay, Andres Gerardo; Simorre, Jean-Pierre; Ebel, Christine; Sapay, Nicolas; Renisio, Jean-Guillaume; Bockmann, Anja; Steinmann, Eike; Pietschmann, Thomas; Dubuisson, Jean; Chipot, Christophe; Penin, Francois. "NMR Structure and Ion Channel Activity of the p7 Protein from Hepatitis C Virus." J. Biol. Chem. 285, 31446-31461 (2010).
Assembly members:
p7tm2, polymer, 29 residues, Formula weight is not available
Natural source: Common Name: Hepatitis C virus Taxonomy ID: 11103 Superkingdom: Viruses Kingdom: not available Genus/species: Hepacivirus Hepatitis C virus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
p7tm2: RLVPGAAYALYGVWPLLLLL
LALPPRAYA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 86 |
| 1H chemical shifts | 224 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | p7tm2 | 1 |
Entities:
Entity 1, p7tm2 29 residues - Formula weight is not available
| 1 | ARG | LEU | VAL | PRO | GLY | ALA | ALA | TYR | ALA | LEU | ||||
| 2 | TYR | GLY | VAL | TRP | PRO | LEU | LEU | LEU | LEU | LEU | ||||
| 3 | LEU | ALA | LEU | PRO | PRO | ARG | ALA | TYR | ALA |
Samples:
sample_1: p7tm2 1 ± 0.2 mM; trifluoroethanol 90%; H2O 10%
sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMR, Varian - collection
NMR spectrometers:
- Varian UnityPlus 500 MHz
- Varian INOVA 800 MHz