BMRB Entry 16014
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16014
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Title: Structure of protein complex PubMed: 19214187
Deposition date: 2008-10-30 Original release date: 2009-02-16
Authors: Wojciak, Jonathan; Martinez-Yamout, Maria; Dyson, Helen; Wright, Peter
Citation: Wojciak, Jonathan; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter. "Structural basis for recruitment of CBP/p300 coactivators by STAT1 and STAT2 transactivation domains" EMBO J. 28, 948-958 (2009).
Assembly members:
TAZ1, polymer, 100 residues, Formula weight is not available
STAT2, polymer, 57 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
TAZ1: ATGPTADPEKRKLIQQQLVL
LLHAHKCQRREQANGEVRAC
SLPHCRTMKNVLNHMTHCQA
GKACQVAHCASSRQIISHWK
NCTRHDCPVCLPLKNASDKR
STAT2: GSHMVPEPDLPHDLRHLNTE
PMEIFRNSVKIEEIMPNGDP
LLAGQNTVDEVYVSRPS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 515 |
| 15N chemical shifts | 145 |
| 1H chemical shifts | 1059 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TAZ1 | 1 |
| 2 | STAT2 | 2 |
| 3 | ZINC ION_1 | 3 |
| 4 | ZINC ION_2 | 3 |
| 5 | ZINC ION_3 | 3 |
Entities:
Entity 1, TAZ1 100 residues - Formula weight is not available
| 1 | ALA | THR | GLY | PRO | THR | ALA | ASP | PRO | GLU | LYS | |
| 2 | ARG | LYS | LEU | ILE | GLN | GLN | GLN | LEU | VAL | LEU | |
| 3 | LEU | LEU | HIS | ALA | HIS | LYS | CYS | GLN | ARG | ARG | |
| 4 | GLU | GLN | ALA | ASN | GLY | GLU | VAL | ARG | ALA | CYS | |
| 5 | SER | LEU | PRO | HIS | CYS | ARG | THR | MET | LYS | ASN | |
| 6 | VAL | LEU | ASN | HIS | MET | THR | HIS | CYS | GLN | ALA | |
| 7 | GLY | LYS | ALA | CYS | GLN | VAL | ALA | HIS | CYS | ALA | |
| 8 | SER | SER | ARG | GLN | ILE | ILE | SER | HIS | TRP | LYS | |
| 9 | ASN | CYS | THR | ARG | HIS | ASP | CYS | PRO | VAL | CYS | |
| 10 | LEU | PRO | LEU | LYS | ASN | ALA | SER | ASP | LYS | ARG |
Entity 2, STAT2 57 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | VAL | PRO | GLU | PRO | ASP | LEU | ||||
| 2 | PRO | HIS | ASP | LEU | ARG | HIS | LEU | ASN | THR | GLU | ||||
| 3 | PRO | MET | GLU | ILE | PHE | ARG | ASN | SER | VAL | LYS | ||||
| 4 | ILE | GLU | GLU | ILE | MET | PRO | ASN | GLY | ASP | PRO | ||||
| 5 | LEU | LEU | ALA | GLY | GLN | ASN | THR | VAL | ASP | GLU | ||||
| 6 | VAL | TYR | VAL | SER | ARG | PRO | SER |
Entity 3, ZINC ION_1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: TAZ1, [U-100% 15N], 1 mM; STAT2; ZINC_ION; H2O 93%; D2O 7%
sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 305 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker Avance 900 MHz
Related Database Links:
| BMRB | 18650 |
| PDB | |
| DBJ | BAE06125 BAG65526 BAI45616 |
| EMBL | CDQ85159 |
| GB | AAB28651 AAC08447 AAC51331 AAC51770 AAH44677 |
| PRF | 1923401A |
| REF | NP_001020603 NP_001073315 NP_001088637 NP_001157494 NP_001247644 |
| SP | P45481 Q6JHU9 Q92793 |
| TPG | DAA15549 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts