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BMRB Entry 16032

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16032
MolProbity Validation Chart

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Title: Nav1.2 C-terminal EF-Hand Domain   PubMed: 19129176

Deposition date: 2008-11-15 Original release date: 2009-01-20

Authors: Miloushev, Vesselin; Levine, Joshua; Arbing, Mark; Hunt, John; Pitt, Geoff; Palmer, Arthur

Citation: Miloushev, Vesselin; Levine, Joshua; Arbing, Mark; Hunt, John; Pitt, Geoff; Palmer, Arthur. "Solution structure of the NaV1.2 C-terminal EF-hand domain."  J. Biol. Chem. 284, 6446-6454 (2009).

Assembly members:
NaV1.2 C-terminal EF-hand domain, polymer, 129 residues, 12130.777 Da.

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
NaV1.2 C-terminal EF-hand domain: MGSSHHHHHHSSGLVPRGSH MASENFSVATEESAEPLSED DFEMFYEVWEKFDPDATQFI EFAKLSDFADALDPPLLIAK PNKVQLIAMDLPMVSGDRIH CLDILFAFTKRVLGESGEMD ALRIQMEER

Data sets:
Data typeCount
13C chemical shifts344
15N chemical shifts101
1H chemical shifts751

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NaV1.2 C-terminal EF-hand domain1

Entities:

Entity 1, NaV1.2 C-terminal EF-hand domain 129 residues - 12130.777 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METALASERGLUASNPHESERVALALATHR
4   GLUGLUSERALAGLUPROLEUSERGLUASP
5   ASPPHEGLUMETPHETYRGLUVALTRPGLU
6   LYSPHEASPPROASPALATHRGLNPHEILE
7   GLUPHEALALYSLEUSERASPPHEALAASP
8   ALALEUASPPROPROLEULEUILEALALYS
9   PROASNLYSVALGLNLEUILEALAMETASP
10   LEUPROMETVALSERGLYASPARGILEHIS
11   CYSLEUASPILELEUPHEALAPHETHRLYS
12   ARGVALLEUGLYGLUSERGLYGLUMETASP
13   ALALEUARGILEGLNMETGLUGLUARG

Samples:

13C-15N: entity, [U-99% 13C; U-99% 15N], 0.5 ± 0.05 mM; glycine, [D5-98%], 100 ± 10 mM; TRIS, [D11-98 %], 20 ± 2 mM; EDTA, [D16-98%], 0.1 ± 0.01 mM; D2O, [U-99% 2H], 10 ± 1 %; DTT, [D10-98%], 1 ± 0.1 mM; NaN3 0.02 ± 0.002 %

10%13-C: entity, [U-10% 13C; U-99% 15N], 0.5 ± 0.05 mM; glycine, [D5-98%], 100 ± 10 mM; TRIS, [D11-98 %], 20 ± 2 mM; EDTA, [D16-98%], 0.1 ± 0.01 mM; D2O, [U-99% 2H], 10 ± 1 %; DTT, [D10-98%], 1 ± 0.1 mM; NaN3 0.02 ± 0.002 %

phage: entity, [U-99% 13C; U-99% 15N], 0.5 ± 0.05 mM; glycine, [D5-98%], 100 ± 10 mM; TRIS, [D11-98 %], 20 ± 2 mM; EDTA, [D16-98%], 0.1 ± 0.01 mM; D2O, [U-99% 2H], 10 ± 1 %; DTT, [D10-98%], 1 ± 0.1 mM; NaN3 0.02 ± 0.002 %; pF1 phage 15 ± 1 mg

sample_conditions: ionic strength: 0.1 M; pH: 7.4; pressure: 1.0 atm; temperature: 290.5 K

Experiments:

NameSampleSample stateSample conditions
3D HNCO13C-15Nisotropicsample_conditions
3D HNCA13C-15Nisotropicsample_conditions
3D HN(CO)CA13C-15Nisotropicsample_conditions
3D HN(CA)CO13C-15Nisotropicsample_conditions
3D HNCACB13C-15Nisotropicsample_conditions
3D CBCA(CO)NH13C-15Nisotropicsample_conditions
3D HCCH-TOCSY13C-15Nisotropicsample_conditions
3D HBHA(CO)NH13C-15Nisotropicsample_conditions
3D 1H-15N NOESY13C-15Nisotropicsample_conditions
3D 1H-13C NOESY13C-15Nisotropicsample_conditions
2D 1H-13C HSQC10%13-Cisotropicsample_conditions
3D HCACOphageanisotropicsample_conditions
2D IPAP HSQCphageanisotropicsample_conditions
3D quant HNCOphageanisotropicsample_conditions
3D CCa HNCOphageanisotropicsample_conditions

Software:

NMRPipe v2.4, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.110, Goddard - chemical shift assignment, peak picking

ARIA v2.2, Linge, O, . - initial structure solution

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - initial structure solution

X-PLOR NIH v2.18, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAA92594 BAC27748 BAC30078 BAG63295 BAK62861
EMBL CAA27287 CAA46438 CAA68735 CAB85895
GB AAA18895 AAA67695 AAB22226 AAG53412 AAG53413
PIR S29185
PRF 1204264B
REF NP_001035232 NP_001035233 NP_001075145 NP_001075146 NP_001092768
SP P04775 P08104 Q99250 Q9NY46
TPG DAA32692 DAA32708 DAA32709 DAA32710 DAA32711

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts