BMRB Entry 16073
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16073
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of cycloviolacin O2 PubMed: 19720036
Deposition date: 2008-12-21 Original release date: 2009-09-04
Authors: Wang, Conan
Citation: Wang, Conan; Colgrave, Michelle; Ireland, David; Kaas, Quentin; Craik, David. "Despite a conserved cystine knot motif, different cyclotides have different membrane binding modes" Biophys. J. 97, 1471-1481 (2009).
Assembly members:
cycloviolacin O2, polymer, 30 residues, Formula weight is not available
Natural source: Common Name: Viola odorata Taxonomy ID: 97441 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Viola odorata
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
cycloviolacin O2: GIPCGESCVWIPCISSAIGC
SCKSKVCYRN
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 181 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cycloviolacin O2 | 1 |
Entities:
Entity 1, cycloviolacin O2 30 residues - Formula weight is not available
| 1 | GLY | ILE | PRO | CYS | GLY | GLU | SER | CYS | VAL | TRP | |
| 2 | ILE | PRO | CYS | ILE | SER | SER | ALA | ILE | GLY | CYS | |
| 3 | SER | CYS | LYS | SER | LYS | VAL | CYS | TYR | ARG | ASN |
Samples:
sample_1: entity 2 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger A. T. et.al. - refinement
NMR spectrometers:
- Bruker DMX 750 MHz