BMRB Entry 16082
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16082
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Title: solution structure of FAPP1 PH domain PubMed: 21454700
Deposition date: 2008-12-22 Original release date: 2010-01-07
Authors: Lenoir, Marc; Coskun, Uenal; James, Jonathan; Simons, Kai; Overduin, Michael
Citation: He, Ju; Scott, Jordan; Heroux, Annie; Roy, Siddhartha; Lenoir, Marc; Overduin, Michael; Stahelin, Robert; Kutateladze, Tatiana. "Molecular basis of phosphatidylinositol 4-phosphate and ARF1 GTPase recognition by the FAPP1 pleckstrin homology (PH) domain." J. Biol. Chem. 286, 18650-18657 (2011).
Assembly members:
FAPP1-PH, polymer, 108 residues, 12219.981 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
FAPP1-PH: GPLGSPEFMEGVLYKWTNYL
TGWQPRWFVLDNGILSYYDS
QDDVCKGSKGSIKMAVCEIK
VHSADNTRMELIIPGEQHFY
MKAVNAAERQRWLVALGSSK
ASLTDTRT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 465 |
| 15N chemical shifts | 117 |
| 1H chemical shifts | 746 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FAPP1-PH | 1 |
Entities:
Entity 1, FAPP1-PH 108 residues - 12219.981 Da.
| 1 | GLY | PRO | LEU | GLY | SER | PRO | GLU | PHE | MET | GLU | ||||
| 2 | GLY | VAL | LEU | TYR | LYS | TRP | THR | ASN | TYR | LEU | ||||
| 3 | THR | GLY | TRP | GLN | PRO | ARG | TRP | PHE | VAL | LEU | ||||
| 4 | ASP | ASN | GLY | ILE | LEU | SER | TYR | TYR | ASP | SER | ||||
| 5 | GLN | ASP | ASP | VAL | CYS | LYS | GLY | SER | LYS | GLY | ||||
| 6 | SER | ILE | LYS | MET | ALA | VAL | CYS | GLU | ILE | LYS | ||||
| 7 | VAL | HIS | SER | ALA | ASP | ASN | THR | ARG | MET | GLU | ||||
| 8 | LEU | ILE | ILE | PRO | GLY | GLU | GLN | HIS | PHE | TYR | ||||
| 9 | MET | LYS | ALA | VAL | ASN | ALA | ALA | GLU | ARG | GLN | ||||
| 10 | ARG | TRP | LEU | VAL | ALA | LEU | GLY | SER | SER | LYS | ||||
| 11 | ALA | SER | LEU | THR | ASP | THR | ARG | THR |
Samples:
sample_1: FAPP1-PH, [U-13C; U-15N], 600 uM; TRIS 20 mM; sodium azide 1 mM; sodium chloride 100 mM
sample_2: FAPP1-PH, [U-15N], 600 uM; TRIS 20 mM; sodium azide 1 mM; sodium chloride 100 mM
sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA v2, Linge, O, . - structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| BMRB | 19508 19576 |
| PDB | |
| DBJ | BAG37067 |
| GB | AAG15199 AAG15200 AAH31110 AAH44567 AAH83759 |
| REF | NP_001013095 NP_001095383 NP_001230393 NP_061964 NP_112546 |
| SP | Q9ERS4 Q9HB20 |
| TPG | DAA32821 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
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or all simulated shifts