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Biological Magnetic Resonance Data Bank


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BMRB Entry 16110

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16110
MolProbity Validation Chart

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Title: Solution structure of the conserved C-terminal dimerization domain of Borealin   PubMed: 19530738

Deposition date: 2009-01-06 Original release date: 2009-06-25

Authors: Lingel, Andreas; Fairbrother, Wayne

Citation: Bourhis, Eric; Lingel, Andreas; Phung, Qui; Fairbrother, Wayne; Cochran, Andrea. "Phosphorylation of a Borealin dimerization domain is required for proper chromosome segregation"  Biochemistry 48, 6783-6793 (2009).

Assembly members:
Borealin, polymer, 76 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Borealin: GSAGERIYNISGNGSPLADS KEIFLTVPVGGGESLRLLAS DLQRHSIAQLDPEALGNIKK LSNRLAQICSSIRTHK

Data sets:
Data typeCount
13C chemical shifts225
15N chemical shifts68
1H chemical shifts465

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_21

Entities:

Entity 1, entity_1 76 residues - Formula weight is not available

1   GLYSERALAGLYGLUARGILETYRASNILE
2   SERGLYASNGLYSERPROLEUALAASPSER
3   LYSGLUILEPHELEUTHRVALPROVALGLY
4   GLYGLYGLUSERLEUARGLEULEUALASER
5   ASPLEUGLNARGHISSERILEALAGLNLEU
6   ASPPROGLUALALEUGLYASNILELYSLYS
7   LEUSERASNARGLEUALAGLNILECYSSER
8   SERILEARGTHRHISLYS

Samples:

unlabeled_sample: Borealin 0.6 mM; sodium phosphate 40 mM; sodium chloride 100 mM; D2O 100%

15N_sample: Borealin, [U-100% 15N], 0.8 mM; sodium phosphate 40 mM; sodium chloride 100 mM; H2O 95%; D2O 5%

15N_13C_sample: Borealin, [U-100% 13C; U-100% 15N], 0.7 mM; sodium phosphate 40 mM; sodium chloride 100 mM; H2O 95%; D2O 5%

15N_13C_sample_D2O: Borealin, [U-100% 13C; U-100% 15N], 0.7 mM; sodium phosphate 40 mM; sodium chloride 100 mM; D2O 100%

mixed_sample: Borealin, [U-100% 13C; U-100% 15N], 0.4 mM; Borealin 0.4 mM; sodium phosphate 40 mM; sodium chloride 100 mM; D2O 100%

sample_conditions_1: ionic strength: 0.1 M; pH: 7.2; pressure: 1 atm; temperature: 299 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESY15N_sampleisotropicsample_conditions_1
3D 1H-13C NOESY15N_13C_sample_D2Oisotropicsample_conditions_1
3D HNHA15N_sampleisotropicsample_conditions_1
2D 1H-1H NOESYunlabeled_sampleisotropicsample_conditions_1
2D 1H-15N HSQC15N_sampleanisotropicsample_conditions_1
3D 1H-13C NOESYmixed_sampleisotropicsample_conditions_1
3D HNCA15N_13C_sampleisotropicsample_conditions_1
3D HNCACB15N_13C_sampleisotropicsample_conditions_1
3D CBCA(CO)NH15N_13C_sampleisotropicsample_conditions_1
3D C(CO)NH15N_13C_sampleisotropicsample_conditions_1
3D H(CCO)NH15N_13C_sampleisotropicsample_conditions_1
3D 1H-15N TOCSY15N_13C_sample_D2Oisotropicsample_conditions_1
2D 1H-15N HSQC15N_sampleisotropicsample_conditions_1
2D 1H-13C HSQC15N_13C_sampleisotropicsample_conditions_1

Software:

TOPSPIN, Bruker - collection

CCPN, CCPN - data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

ARIA v2.2, Linge, O, . - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

Molmol, Koradi, Billeter and Wuthrich - Display

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
DBJ BAA91629 BAB13961 BAB14125 BAD96269 BAD96288
EMBL CAH90785
GB AAH00703 AAH01651 AAH08079 AAH16944 AAR91699
REF NP_001127334 NP_001233359 NP_001243804 NP_060571 XP_001115347
SP Q53HL2 Q5RBS5

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