BMRB Entry 16153
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16153
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Title: Structure of the transcription repressor SvtR08 (gp08) from the hyperthermophilic archaeal virus SIRV1 PubMed: 19535331
Deposition date: 2009-01-30 Original release date: 2009-06-26
Authors: Guilliere, Florence; Kessler, Alexandra; Peixeiro, Nuno; Sezonov, Guennadi; Prangishvili, David; Delepierre, Muriel; Guijarro, J. Inaki
Citation: Guilliere, Florence; Peixeiro, Nuno; Kessler, Alexandra; Raynal, Bertrand; Desnoues, Nicole; Keller, Jenny; Delepierre, Muriel; Prangishvili, David; Sezonov, Guennadi; Guijarro, J. Inaki. "Structure, function and targets of the transcriptional regulator SvtR from the hyperthermophilic archaeal virus SIRV1." J. Biol. Chem. 284, 22222-22237 (2009).
Assembly members:
svtr08, polymer, 56 residues, 6620.74 Da.
Natural source: Common Name: Sulfolobus virus SIRV1 Taxonomy ID: 157898 Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
svtr08: MQTQEQSQKKKQKAVFGIYM
DKDLKTRLKVYCAKNNLQLT
QAIEEAIKEYLQKRNG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 261 |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 425 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 1 |
Entities:
Entity 1, entity_1 56 residues - 6620.74 Da.
Residues 1-12 are disordered. Only coordinates for residues 11-56 are included. Final structures were calculated with data for residues 11-56
| 1 | MET | GLN | THR | GLN | GLU | GLN | SER | GLN | LYS | LYS | ||||
| 2 | LYS | GLN | LYS | ALA | VAL | PHE | GLY | ILE | TYR | MET | ||||
| 3 | ASP | LYS | ASP | LEU | LYS | THR | ARG | LEU | LYS | VAL | ||||
| 4 | TYR | CYS | ALA | LYS | ASN | ASN | LEU | GLN | LEU | THR | ||||
| 5 | GLN | ALA | ILE | GLU | GLU | ALA | ILE | LYS | GLU | TYR | ||||
| 6 | LEU | GLN | LYS | ARG | ASN | GLY |
Samples:
sample_1: svtr08 1.6 mM; sodium acetate, [U-2H], 20 mM; D2O 10%; H2O 90%
sample_2: svtr08 1.2 mM; sodium acetate, [U-2H], 20 mM; D2O 100%
sample_3: svtr08, [U-98% 13C; U-98% 15N], 1.5 mM; sodium acetate, [U-2H], 20 mM; D2O 12%; H2O 88%
sample_4: svtr08, 50%[U-98% 13C; U-98% 15N]; 50% natural abundance, 2.9 mM; sodium acetate, [U-2H], 20 mM; D2O 12%; H2O 88%
sample_5: svtr08, [U-98% 15N], 1.7 mM; sodium acetate, [U-2H], 20 mM; D2O 10%; H2O 90%
sample_conditions_1: pH: 5.5; pressure: 1.0 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_3 | isotropic | sample_conditions_1 |
| 3D DOUBLY-FILTERED 1H-13C NOESY | sample_4 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_5 | isotropic | sample_conditions_1 |
Software:
ARIA v2.2, Linge, O, . - nOe assignment, structure solution
CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution
VNMRJ v2.1B, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView v5.2.2, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking
ProcheckNMR v3.4, Laskowski and MacArthur - data analysis
WhatIF, Vriend - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 500 MHz
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