BMRB

Biological Magnetic Resonance Data Bank


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BMRB Entry 16233

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16233
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Title: 1H, 13C, 15N Chemical Shift Assignments for Eimeria tenella microneme protein 3 mar1a domain   PubMed: 19888684

Deposition date: 2009-03-30 Original release date: 2009-05-20

Authors: Lai, Livia; Matthews, Stephen; Simpson, Pete

Citation: Lai, Livia; Simpson, Pete; Bumstead, Janine; Tomley, Fiona; Matthews, Stephen. "Complete NMR assignments for the second microneme adhesive repeat (MAR) domain from Eimeria tenella microneme protein EtMIC3"  Biomol. NMR Assign. 3, 175-177 (2009).

Assembly members:
mar1a, polymer, 123 residues, Formula weight is not available

Natural source:   Common Name: Eimeria tenella   Taxonomy ID: 5802   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Eimeria tenella

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
mar1a: APTLQEALDRKCAQFGKESC NSGLASYCGAAIYARYDKGN SSQQTKEWRCYIEKELDFSK SGDGCVDDCGSMTSCRGSVS GPSTSHLTRPSELQAVIDSD KSNYCTDKSEQQPSQATPPP EIP

Data sets:
Data typeCount
13C chemical shifts481
15N chemical shifts121
1H chemical shifts676

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1mar1a1

Entities:

Entity 1, mar1a 123 residues - Formula weight is not available

1   ALAPROTHRLEUGLNGLUALALEUASPARG
2   LYSCYSALAGLNPHEGLYLYSGLUSERCYS
3   ASNSERGLYLEUALASERTYRCYSGLYALA
4   ALAILETYRALAARGTYRASPLYSGLYASN
5   SERSERGLNGLNTHRLYSGLUTRPARGCYS
6   TYRILEGLULYSGLULEUASPPHESERLYS
7   SERGLYASPGLYCYSVALASPASPCYSGLY
8   SERMETTHRSERCYSARGGLYSERVALSER
9   GLYPROSERTHRSERHISLEUTHRARGPRO
10   SERGLULEUGLNALAVALILEASPSERASP
11   LYSSERASNTYRCYSTHRASPLYSSERGLU
12   GLNGLNPROSERGLNALATHRPROPROPRO
13   GLUILEPRO

Samples:

mar1a_H2O: mar1a, [U-13C; U-15N], 0.5 mM; H2O 90%; D2O 10%; sodium phosphate 20 mM; sodium chloride 50 mM

mar1a_D2O: mar1a, [U-13C; U-15N], 0.5 mM; D2O 100%; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCmar1a_H2Oisotropicsample_conditions_1
2D 1H-13C HSQCmar1a_H2Oisotropicsample_conditions_1
3D HNCACBmar1a_H2Oisotropicsample_conditions_1
3D CBCA(CO)NHmar1a_H2Oisotropicsample_conditions_1
3D HN(CO)CAmar1a_H2Oisotropicsample_conditions_1
3D HNCOmar1a_H2Oisotropicsample_conditions_1
3D HBHA(CO)NHmar1a_H2Oisotropicsample_conditions_1
3D HCCH-TOCSYmar1a_D2Oisotropicsample_conditions_1
3D H(CCO)NHmar1a_H2Oisotropicsample_conditions_1
3D 1H-15N NOESYmar1a_H2Oisotropicsample_conditions_1
3D 1H-13C NOESYmar1a_D2Oisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker Avance 800 MHz

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