BMRB Entry 16243

Name: Backbone NH Assignments for Prp24-RRM23
Published: 2009-09-04

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PDB ID: 2kh9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16243
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone NH Assignments for Prp24-RRM23 PubMed: 19693704

Deposition date: 2009-04-07 Original release date: 2009-09-04

Authors: Martin-Tumasz, Stephen; Butcher, Samuel

Citation: Martin-Tumasz, Stephen; Butcher, Samuel. "(1)H, (13)C and (15)N resonance assignments of a ribonucleoprotein complex consisting of Prp24-RRM2 bound to a fragment of U6 RNA." Biomol. NMR Assignments 3, 227-230 (2009).

Assembly members:
Prp24-RRM23, polymer, 186 residues, 21430 Da.

Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
Prp24-RRM23: MTECTLWMTNFPPSYTQRNI RDLLQDINVVALSIRLPSLR FNTSRRFAYIDVTSKEDARY CVEKLNGLKIEGYTLVTKVS NPLEKSKRTDSATLEGREIM IRNLSTELLDENLLRESFEG FGSIEKINIPAGQKEHSFNN CCAFMVFENKDSAERALQMN RSLLGNREISVSLADKKPLE HHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	158
15N chemical shifts	158

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Prp24-RRM23	1

Entities:

Entity 1, Prp24-RRM23 186 residues - 21430 Da.

Final 8 residues are non-native due to 6x His tag

1

MET

THR

GLU

CYS

THR

LEU

TRP

MET

THR

ASN

2

PHE

PRO

SER

TYR

THR

GLN

ARG

ASN

ILE

3

ARG

ASP

LEU

GLN

ASP

ILE

ASN

VAL

4

ALA

LEU

SER

ILE

ARG

LEU

PRO

SER

LEU

ARG

5

PHE

ASN

THR

SER

ARG

PHE

ALA

TYR

ILE

6

ASP

VAL

THR

SER

LYS

GLU

ASP

ALA

ARG

TYR

7

CYS

VAL

GLU

LYS

LEU

ASN

GLY

LEU

LYS

ILE

8

GLU

GLY

TYR

THR

LEU

VAL

THR

LYS

VAL

SER

9

ASN

PRO

LEU

GLU

LYS

SER

LYS

ARG

THR

ASP

10

SER

ALA

THR

LEU

GLU

GLY

ARG

GLU

ILE

MET

11

ILE

ARG

ASN

LEU

SER

THR

GLU

LEU

ASP

12

GLU

ASN

LEU

ARG

GLU

SER

PHE

GLU

GLY

13

PHE

GLY

SER

ILE

GLU

LYS

ILE

ASN

ILE

PRO

14

ALA

GLY

GLN

LYS

GLU

HIS

SER

PHE

ASN

15

CYS

ALA

PHE

MET

VAL

PHE

GLU

ASN

LYS

16

ASP

SER

ALA

GLU

ARG

ALA

LEU

GLN

MET

ASN

17

ARG

SER

LEU

GLY

ASN

ARG

GLU

ILE

SER

18

VAL

SER

LEU

ALA

ASP

LYS

PRO

LEU

GLU

19

HIS

Samples:

Fully_Labeled: Prp24-RRM23, [U-99% 13C; U-99% 15N], 500 mM; TRIS 10 mM; potassium chloride 50 mM; DTT 1 mM; H20 90%; D20 10%

Referencing: Prp24-RRM23, [U-99% 13C; U-99% 15N], 500 uM; TRIS 10 mM; potassium chloride 50 mM; DTT 1 mM; DSS 10 uM; H20 90%; D20 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	Fully_Labeled	isotropic	sample_conditions_1
3D CBCA(CO)NH	Fully_Labeled	isotropic	sample_conditions_1
3D HNCACB	Fully_Labeled	isotropic	sample_conditions_1
3D HNCA	Fully_Labeled	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	16244 17589
PDB	2GHP 4N0T
DBJ	GAA25719
EMBL	CAA89251 CAY82100
GB	AAU09775 AHY76723 AJP40962 AJS62134 AJS62569
REF	NP_013995
SP	P49960
TPG	DAA10168

Biological Magnetic Resonance Data Bank